HPLC测定伸舒活乳膏剂中总阿魏酸的含量  

Determination of total ferulic acid in Shenshuhuo cream by HPLC

作　　者：梁栗[1] 侯世祥[1] 王觉晓[1] 王高森[1] 黄利[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2007年第1期88-90,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的 建立测定伸舒活乳膏剂中总阿魏酸含量的方法。方法 采用HPLC法，用C18柱（150mm×4．6mm，5μm）；流动相为甲醇-2％冰醋酸（32：68）；检测波长320nm；柱温30％：流速1ml·min^-1。结果 阿魏酸在4．55—91．00μg·ml。范围内线性关系良好（r^2=0．9998），方法 回收率为98．78％，RSD=2．67％（n=9）。结论 所建方法快速、准确、重复性好，适用于制剂的质量控制。OBJECTIVE To establish an HPLC for determining total ferulic acid in Shenshuhuo creams. METHODS Diamonsil C 18 column （ 150 mm × 4.6 mm,5 μm） was used. The mobile phase consisted of methyl alcohol -2% acetic acid（ 32 ： 68）. The detection wavelength was set at 320 nm. The column temperature was 30℃. The flow rate of the mobile phase was 1.0 ml· min ^-1. RESULTS The linear range of ferulic acid was in the range of 4.55 -91.00 μg·ml^-1（ r^2 =0.9998）. The average recovery was 98.78% with the RSD of 2.67% （ n = 9）. CONCLUSION The method is rapid, highly accurate, precise and reproducable. It can be used to control the quality of Shenshuhuo cream.

关 键 词：伸舒活乳膏剂 总阿魏酸 高效液相色谱法 含量测定 

分 类 号：R297[医药卫生—民族医学]