苜蓿雄性不育系及其回交后代同工酶分析  

Analysis of Isozymes in Male Sterile line and Its Backcross Progenies of Alfalfa

作　　者：孙静[1] 石凤翎[1] 高翠萍[1] 杜宝红[1] 李泽江[1] 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010019

出　　处：《种子》2007年第7期34-40,共7页Seed

基　　金：国家自然科学基金(30160057);内蒙古自然科学基金(050303-2)。

摘　　要：采用聚丙烯酰胺凝胶垂直板电泳技术对苜蓿雄性不育系及其回交后代进行过氧化物酶(POD)、酯酶(EST)同工酶分析。结果表明:(1)苜蓿雄性不育系及其回交后代叶的POD同工酶在现蕾期和开花期的酶带数较返青期多。返青期的酶活性较现蕾期和开花期强;MS-4及其回交后代叶的EST同工酶活性变化规律与POD同工酶谱表现相似;(2)在花发育的不同时期,花蕾为绿色时期时的POD同工酶酶活性最强,MS-4及其回交后代小花的EST同_T-酶酶带数较叶的多。刚开过的小花EST同工酶酶活性最强,F1、BC1代不育株都出现了母本MS-4所具有的POD、EST特征酶带;(3)从MS-4回交后代中所选育的不育株的POD和EST同工酶谱明显偏向于轮回亲本MS-4,说明通过回交使MS-4的某些性状在回交后代中进一步得到表现,因此,可以初步认定原不育系Ms4的不育性是可遗传的。By means of vertical slab polyaerylamide gel electrophoresis, perioxidase isozyme （POD）and esterase isozyme（EST） in male sterile line and its baekeross progenies of Alfalfa were analyed. The results showed that, （1） The POD isozyme bands number of leaves of male sterile line and its baekeross progenies in Alfalfa at budding period and flowering period are more than that at turning green period, the isozyme activity at turning green period is higher than that at budding period and flowering period. And the change law in the isozyme activity of EST is the same as that of POD. （2）At the different developing period of flowers, the isozyme activity of EST at green bud period is highest. The EST isozyme bands number of flowers is more than that of leaves, and the isozyme activity of EST of flowers that have bloomed are highest. The F1 and BC1 sterile plants have the characteristic POD and EST isozymes bands in MS-4. （3） The pattern of POD and EST isozymes of the sterile plants that were selected from backcross progenies is obviously similar to recurrent parent MS-4, which showed that some qualities of MS-4 has further appeared in backcross progenies. Therefore, we can identify that the sterility of MS-4 can inherit.

关 键 词：苜蓿 雄性不育系 回交后代 过氧化物同工酶 酯酶同工酶 

分 类 号：S551.7[农业科学—作物学]