弥漫性脑损伤后肠黏膜免疫功能的改变  

The changes of the rat intestinal mucosal immunity following diffuse brain injury

作　　者：赵希敏 刘霞 沈晓燕 

机构地区：[1]上海宝山中心医院神经外科,201900

出　　处：《中华神经外科杂志》2007年第7期503-506,共4页Chinese Journal of Neurosurgery

基　　金：上海市科委科研基金资助项目(034119942)

摘　　要：目的探讨弥漫性脑损伤后肠道免疫功能的改变。方法应用大鼠弥漫性脑损伤模型。SD大鼠80只,分为健康对照组20只;弥漫性脑损伤组60只,再分成3个亚组(24h,3d,7d)。检测大鼠胆汁中sIgA、小肠上皮内淋巴细胞的T细胞及其亚群,观察肠黏膜的病理改变以及血液内毒素的变化。结果颅脑创伤后大鼠胆汁中sIgA相对于对照组明显减少(P<0.01),损伤后7d仍低于正常水平(P=0.139);颅脑创伤后24h大鼠小肠黏膜CD4+显著减少(P<0.01),CD8+变化不明显, CD4+/CD8+显著降低(P<0.01);颅脑创伤组静脉血内毒素均高于对照组(P<0.01),病理学检查:颅脑创伤后肠黏膜受损,肠绒毛高度在大鼠脑损伤后减少(P<0.01)。结论颅脑创伤导致肠道免疫功能下降从而可能引发多器官功能障碍。Objective To investigate changes of the rat intestinal mucosal immunity following diffuse brain injury（DBI）. Methods Eighty SD rats were divided randomly into control group（n = 20）and DBI group. The animal model established by Marmarou was used to produce DBI. Rats of the DBI group were sacrificed at 24h, 3d and 7d（ n =20）. The changes of intestinal intraepithelial lymphocyte, slgA in bile, endotoxin in vein blood and pathological changes in intestines were investigated. Results The concentration of slgA in bile of the DBI group was reduced significantly. The concentration of slgA in bile of DBI group was lower than that in control group. There was a significant reduction in the number of CD4 ^＋ T lymphocytes in rat intestines of the DBI group. The number of CD8 ^＋ T lymphocytes in rat intestines of the DBI group did not increased, and the ratio of CD4 ^＋ to CD8 ^＋ was decreased. The endotoxin of vein blood of the DBI group from 24h to 7d was higher than that in the control group. Intestinal mucosa was damaged, the intestinal villi of the DBI was shorter than that of the control group. Conclusion Diffuse brain injury causes intestinal immunity dysfunction, and may lead to multiple organ dysfunction.

关 键 词：颅脑创伤 肠黏膜 免疫 内毒素 大鼠 

分 类 号：R651.15[医药卫生—外科学]