基于电聚合硫堇膜与纳米复合材料界面的直接电化学免疫传感器的构建  

Design of a Direct Electrochemical Immunosensor Based on Redox Electropolymerized Poly(Thionine) Film and Composite Nanomaterials

作　　者：冯辉[1] 王桦[1] 张云[1] 严八妮[1] 张玉玮[1] 

机构地区：[1]湖南大学化学化工学院化学/生物传感与计量学国家重点实验室

出　　处：《传感技术学报》2007年第8期1683-1686,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助项目(20435010;20375012)

摘　　要：本工作采用电聚合手段在玻碳电极表面制备聚硫堇基质膜,借硫堇膜上富含的氨基基团实现对碳纳米管与纳米金颗粒二元纳米复合材料的化学组装,制备稳定的碳纳米管/纳米金/聚硫堇传感界面,以此传感界面固定免疫活性物质,发展了一种无电子媒介的酶免疫传感器.结果表明,电极采用碳纳米管/纳米金颗粒界面固定免疫活性物质,其传感响应性能明显优于单独采用碳纳米管的情况.以人IgG免疫体系为例,电极以此界面固定人IgG抗体,并通过夹心方式直接响应酶催化H2O2的还原电流信号,实现了对人IgG浓度的灵敏测定.此外,采用甘氨酸-HCl缓冲液洗涤使用后的传感器,可有效洗脱其表面的免疫复合物,实现传感器的重复使用.A reagent-free enzyme-based immunosensor has been fabricated by corporately covalent assembly of car- bon nanotubes （CNT） and gold nanoparticles （GNP） onto the poly（thionine） （PTH） film that was deposited on the glass carbon electrode （GCE） by electro-polymerizatiorL The results showed that better analytical performances were obtained for the as-proposed enzyme immunosensor, comparing with that using CNT only. Moreover, as a model immunological agent system, IgG antibodies were immobilized onto the PTH-CNT-GNP modified electrode to recognize IgG target by the ＂sandwich＂ detection way. The concentrations of human IgG in serum samples were directly determined according to the He 02 reduction currents catalyzed by the enzyme-labeled second antibodies. In addition, the used sensors were regenerated by using 0. 10mol/L glycin-HCl （pH 2. 8） to dissociate the antibody- antigen complex, showing the well repeatable usage of the electrodes.

关 键 词：聚硫堇 碳纳米管 纳米金颗粒 酶免疫传感器 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]