栽培稻-紧穗野生稻双二倍体的产生及其细胞遗传学研究  

The Production and Cytogenetical Studies of Oryza sativa-Oryza eichingeri Amphiploid

作　　者：颜辉煌 熊振民[1] 闵绍楷[1] 胡慧英[1] 张志涛[1] 田淑兰[1] 傅强[1] 

机构地区：[1]中国水稻研究所

出　　处：《Acta Genetica Sinica》1997年第1期30-35,共6页

基　　金：中国农业科学院科学基金;国家自然科学基金

摘　　要：从栽培稻与紧穗野生稻杂种Ｆ１的花培再生植株Ｈ１中，筛选到Ｅ１３和Ｅ２４两个抗褐飞虱的双二倍体，并对其遗传行为进行了研究。与杂种Ｆ１相比，双二倍体表现为植株变矮，每穗颖花数减少，穗长与花药长增加，颖花变大，并具有较低的自交育性，Ｈ１代的结实率分别为２．７２％和３．５５％，Ｈ２代分别为４．２９％和４．７２％。进一步对Ｅ１３和Ｅ２４的花粉母细胞染色体配对情况进行观察，发现两者中期Ｉ染色体构型分别为０．６９Ｉ＋２３．５４Ⅱ＋０．０６Ⅳ和０．５９Ⅰ＋２３．３６Ⅱ＋０．１４Ⅳ。本研究还发现，两个材料的Ｈ２和Ｈ３植株染色体数均为２ｎ＝４８，彼此形态表现相似，说明上述双二倍体具有相对高的遗传稳定性。wo BPH resistant amphiploids,E13 and E24(2n=48),were selected from pollen-derived regenerants (H1) of Oryza sativa×Oeichingeri F1 brid,and their genetic behavior was investigatedThey were characterized by shorter plant height,decreased spikelets per panicle,longer panicle and anther,and larger spikelets,in comparison with the original F1 hybridThey were also self fertile,although somewhat low,with seed set ranging from 272% to 355% in H1 and from 429% to 472% in H2The meiotic analysis further revealed the chromosome configuration at MI of E13 and E24,being 069Ⅰ+2354Ⅱ+006Ⅳ and 059Ⅰ+2336Ⅱ+014Ⅳ,respectivelyThe invariable chromosome numbers(2n=48)and the similar morphological traits in H2 and H3generations led to the conclusion that these two amphiploids had a relatively high genetic stability

关 键 词：水稻 栽培稻 紧穗野生稻 双二倍体 细胞遗传学 

分 类 号：S511.032[农业科学—作物学]