利用钙调素对乳酸脱氢酶活性影响定量测定钙调素  

Quantitative Assay of Calmodulin Based on Its Activation of Lactate Dehydrogenase

作　　者：杜国忠[1] 赵宝昌[1] 

机构地区：[1]大连医科大学生化教研室,大连116027

出　　处：《生物化学杂志》1997年第1期122-124,共3页

摘　　要：Calmodulin (CaM) can activate several CaM-dependent-enzymes in the presence ofCa2+. The activative effect of CaM on lactate dehydrogenase (LDH, EC 1. 1. 1. 27) was studied.LDH was purified from bovine heart and CaM from bovine brain, respectively. Based on its activation of LDH by CaM, a quantitative assay of CaM was developed. Results of kinetic study showthat this activation belongs to "non-competitive activation".Calmodulin (CaM) can activate several CaM-dependent-enzymes in the presence ofCa2+. The activative effect of CaM on lactate dehydrogenase (LDH, EC 1. 1. 1. 27) was studied.LDH was purified from bovine heart and CaM from bovine brain, respectively. Based on its activation of LDH by CaM, a quantitative assay of CaM was developed. Results of kinetic study showthat this activation belongs to 'non-competitive activation'.

关 键 词：调钙蛋白 乳酸脱氢酶 活性 定量测定 

分 类 号：Q513.03[生物学—生物化学] Q554.9