固相萃取-高效液相色谱法同时检测健康人血浆中的异荭草素、荭草素和灯盏乙素浓度  

Simultaneous determination the concentration of isoorientin,orientin and scutellarin in healthy human plasma by solid phase extraction-HPLC

作　　者：张颖[1] 高蕊[2] 刘建勋[1] 王书臣[2] 

机构地区：[1]中国中医科学院西苑医院基础研究中心,北京100091 [2]中国中医科学院西苑医院临床药理中心,北京100091

出　　处：《中国临床药理学杂志》2007年第4期299-303,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家863"临床试验关键技术及平台研究"课题资助项目(2004AA2Z3762)

摘　　要：目的建立快速、准确、灵敏的分析方法检测3种主要活性黄酮成分:异荭草素、荭草素和灯盏乙素(抗冠心病药)在健康人体的血药浓度。方法健康人9例静脉滴注复方荭草冻干粉针后,选择固相萃取方法,在适当条件下一次提取3种成分,流动相:乙腈-四氢呋喃-磷酸缓冲液(pH=2)=9∶6∶85;柱温:40℃;流量:1.0mL.min-1;检测波长:340nm。结果异荭草素、荭草素和灯盏乙素与其他内源性和药源性成分达到良好分离,最低检测浓度分别达到5,5,10ng.mL-1,平均回收率分别为93.0%,86.2%和97.5%。并能在17min内完成样品分析。结论该方法对3种检测成分可特异、准确、快速和灵敏的分析,可用于复方荭草冻干粉针在健康人的血药浓度分析。Objective To study pharmacokinetics of isoorientin, orientin and scutellarin which are major active flavones in compound Hongcao, extract of polygonura orientale L. and Erigeron breviscapus in healthy volunteers. Methods Solidphase extraction procedure was developed to extract three components from human plasma simultaneously. The plasma samples were analyzed on C18 column with the mobile phase composed of acetonitrile - tetrahydrofuran - phosphate buffer （ pH = 2 ） = 9 ： 6 ： 85. The elution was performed at the flow rate of 1.0 mL·min^- 1, detected at 340 nm. Results The analysis of plasma sample could be performed in 17 min with the limit of quantitation of 5,5and 10 ng· mL^-1 and average recovery of 93.0% , 86.2% and 97.5% for isoorientin, orientin and scutellarin, respectively. Conclusion The method was sensitive, accurate, rapid and can be applied to determine the plasma concentration of isoorientin, orientin and scutellarin after single intravenous drip infusion.

关 键 词：异荭草素 荭草素 灯盏乙素 药代动力学 固相萃取-高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学] R972.3[医药卫生—药学]