Nogo抗体治疗脊髓损伤的实验研究  

Treatment of spinal cord injury with Nogo antibodies

作　　者：侯巍[1] 冯世庆[1] 陈家童[2] 王沛[1] 周先虎[1] 荆峰[1] 郭世绂[1] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]南开大学分子生物学研究所

出　　处：《中华骨科杂志》2007年第8期609-613,共5页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金资助项目（30271313）

摘　　要：目的探讨Nogo抗体应用于脊髓损伤动物模型中的疗效。方法压迫法制成Wistar大鼠T10脊髓损伤模型共40例，随机分为两组，实验组采用Nogo抗体治疗，对照组注入生理盐水。术后第1天及4、8、12周，对两组动物的双后肢进行躯体感觉诱发电位（somatosensory evoked potentials，SEP）和运动诱发电位（motor evoked potentials，MEP）检查，记录N1及D波潜伏期和波幅；术后第1天及2、4、6、8、10、12周对动物进行Basso Beatlie Bresnahan（BBB）运动功能评分。术后12周处死动物，取出脊髓组织，冷冻切片后进行HE染色和免疫组化染色，观察神经元中丝改变；神经纤维免疫荧光染色观察神经纤维再生情况，并测量染色阳性面积，综合评估脊髓损伤后功能恢复的程度。结果术后第1天及4、8、12周，实验组SEP-N1潜伏期分别为（38．51±0．70）ms、（37．54±0．47）ms、（35．43±0．30）ms、（34．88±0．27）ms，对照组为（38．76±0．33）ms、（38．55±0．49）ms、（36．61±0．38）ms、（36．06±0．20）ms，差异有统计学意义（P〈0．05）。两组SEP-N1波幅及MEP-D潜伏期和波幅、BBB评分的差异均有统计学意义（P〈0．05）。实验组神经纤维免疫荧光染色阳性面积高于对照组，差异有统计学意义（P〈0．05），实验组的疗效优于对照组。结论Nogo抗体治疗脊髓损伤能促进神经元的再生，恢复脊髓功能。Nogo抗体的获取比较方便，技术操作简便易行，为其真正应用于临床奠定良好的基础。Objective To evaluate the clinical effect of Nogo antibodies on the treatment of spinal cord injury （SCI） of adult rats model. Methods Forty adult compressed injury rats model of the tenth thoracic spinal cord level were used in the study. They were randomly divided into two groups. The experimental group was treated with Nogo antibodies and the control group was treated with saline injection after SCI. The recovery of lower extremities was observed with somatosensory evoked potentials （SEP）, motor evoked potentials （MEP） at 1 d, 4 weeks, 8 weeks, 12 weeks and Basso Beatlie Bresnahan （BBB） score at 1 d, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks after the operation of Nogo antibodies and saline injection. 12 weeks postoperation, these rats were all killed, the spinal cord including the injured site were taken out and cut into slim. The slices were stained by nerve fiber, HE, and immunohistochemistry. The change of nerve fiber, the regeneration of axon and the positive immunofluorescence area of nerve fiber of each group were recorded. Results The potential period of SEP-N1 in the experimental group were： （38.51±0.70） ms 1 d postoperation, （37.54±0.47） ms 4 weeks postoperation, （35.43±0.30） ms 8 weeks postoperation, （34.88±0.27） ms 12 weeks postoperation; the potential period in control group were： （38.76±0.33） ms, （38.55±0.49） ms, （36.61±0.38） ms, （36.06±0.20） ms respectively. There were statistical difference of potential period and voltage of SEP-N1 and MEP-D between two groups （P〈 0.05）. The treatment using Nogo antibodies could promote the electrophysiological and motor functional recovery, its curative effect was better than that in the control group; the positive immunofluorescence area of nerve fiber in the Nogo group was al- so larger than that in the control group （P〈 0.05）. Conclusion Using Nogo antibodies is an effective method of SCI treatment. It can increase nerve axon regeneration of injured sites, recover spinal el

关 键 词：脊髓损伤 再生 轴突 

分 类 号：R686[医药卫生—骨科学]