HPLC法测定银黄颗粒中绿原酸的含量  

Content determination of chlorogenic acid in Yinhuang granules by HPLC

作　　者：刘文丽 

机构地区：[1]安徽六安市药品检验所中药室,安徽六安237006

出　　处：《安徽医药》2007年第8期711-712,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 建立银黄颗粒中绿原酸的含量测定方法.方法 采用高效液相色谱法,色谱柱VP-ODS柱（150 mm×4.6 mm,5 μm）;流动相：乙腈-0.4%磷酸溶液（13∶87）,检测波长327 nm,流速1.0 ml·min^-1,柱温室温.结果 回归方程Y=-1.440×10^2＋1.245×10^6X （r=0.999 9,n=5）,绿原酸在0.88～13.2 μg范围内线性良好,加样回收率99.39%,RSD=0.53%.结论 本法灵敏度高,操作简便,重复性好,准确可靠,可作为银黄颗粒中绿原酸的含量测定方法.Aim To establish a method for the determination of chlorogenic acid in Yinhuang granules.Methods HPLC method was used.The samples were separated by VP-ODS column（150 mm×4.6 mm,5 μm）.The mobile phase is actonitrile-0.4% acetic acid（13∶87）.The detection wavelength was at 327 nm,with flow rate at 1.0 ml·min^-1.The column temperature was indoor temperature.Results The linear range of schisandrin was 0.88～13.2 μg（r=0.999 9,n=5）.The average rescovery was 99.39%,and RSD was 0.53%.Conclusion This method is sensitive,quick,accurate and can be used to determine the contents of chlorogenic acid in Yinhuang granules.

关 键 词：银黄颗粒 HPLC 绿原酸 含量测定 

分 类 号：R286[医药卫生—中药学]