3种杀螨剂生物测定方法的比较研究  

Comparison of Three Acaricide Bioassay Methods

作　　者：张永强[1] 丁伟[1] 吴静[1] 赵志模[1] 

机构地区：[1]西南大学植物保护学院,重庆400716

出　　处：《农药科学与管理》2007年第7期50-53,共4页Pesticide Science and Administration

摘　　要：在杀螨剂研究中,常规的玻片浸渍法或叶片浸渍法都无法满足微量物质的快速测定工作。在玻片浸渍法的基础上,采用玻璃点样毛细管对螨体直接施药,解决了这一问题。文中比较了玻片浸渍法,叶片浸渍法及玻片毛细管法3种测定方法对用4种溶剂和水提取的黄花蒿的提取物和常用杀螨剂哒螨灵对朱砂叶螨的作用效果,结果证实了采用玻片毛细管法测定的结果与玻片浸渍法的结果相近,但其用药量降低了1000倍,这对植物源杀螨剂以及生物农药研究都有重要意义。In acaricides study, common slide dip method or leaf dip method was not met with the rapid acaricidal activity screening of minim substances. Based on the slide dip method, applying directly to mite individually by glass drop-sample capillary would help solve this problem effectively. In this paper, the acarcidal activities of 4 solvents and water extracts of Atemisia annua L. and pyridaben were compared by three bioassay methods, slide dip method, leaf dip method and slide-capillary method in laboratory. The results showed that the effect of slide-capillary method was close to the slide dip method, but the dosage of slide-capillary method was just millesimal of slide dip method, which was significant to the study of botanical acaricide and biopesticide.

关 键 词：玻片毛细管法 杀螨剂 黄花蒿 哒螨灵 

分 类 号：S482.52[农业科学—农药学]