Grp78/Bip介导戊型肝炎病毒衣壳蛋白对宿主细胞的吸附  

Grp78/Bip facilitates the attachment/entry of the hepatitis E virus capsid protein to host cells

作　　者：吴小成[1] 苗季[1] 郑子峥[1] 何水珍[1] 孙媛媛[1] 唐明[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,福建厦门361005

出　　处：《微生物与感染》2007年第2期83-87,共5页Journal of Microbes and Infections

基　　金：福建省科技重大专项(2004y201);福建省青年科技人才创新项目(2006F3124)

摘　　要：目的探讨Grp78/Bip在戊型肝炎病毒(HEV)衣壳蛋白进入宿主细胞过程中的作用。方法采用pull-down和免疫共沉淀技术进一步验证p239与Grp78/Bip的相互作用;采用激光共聚焦显微镜技术检测p239与细胞膜表面Grp78/Bip共定位情况;采用原核表达纯化的Grp78/Bip蛋白封闭p239的Grp78/Bip结合位点,检测其阻断p239吸附细胞的效果。结果p239与Grp78/Bip可以直接结合,而且这种结合是可逆的生理性结合;p239与Grp78/Bip在细胞膜上存在部分共定位;Grp78/Bip能部分阻断p239对肝细胞的吸附。结论Grp78/Bip参与并介导HEV衣壳蛋白对宿主细胞的吸附。Objective To further investigate the interaction between recombinant hepatitis E virus （HEV） capsid protein p239 and Grp78/Bip and the role of Grp78/Bip in HEV penetration. Methods We utilized pull-down, immunoprecipitation and antibody blocking assays to examine the interaction between p239 and Grp78/Bip. Confocal microscopy was used to investigate the co-localization of these two proteins. Purified Grp78/Bip was used to block the attachment of p239 to host cells. Results p239 directly bound to Grp78/Bip and this binding was sensitive to ATP, Furthermore, antibody blocking results demonstrate that this interaction was indeed conformation-dependent. A partial co-localization of p239 and Grp/Bip was observed on the plasma membrane of HepG2 by confocal microscopy, Pre-incubation of Grp78/ Bip with p239 significantly blocked the attachment of p239 to HepG2 cells. Collusion Grp78/Bip participates in the attachment and/or entry of the HEV capsid protein to host cells. These results further contribute to the tmderstanding of the entry mechanism of the hepatitis E virus after infection.

关 键 词：戊型肝炎病毒 衣壳 Grp78/Bip 病毒吸附 

分 类 号：R373[医药卫生—病原生物学]