Liquid Chromatography-Electrospray Quadrupole Linear Ion Trap Mass Spectrometric Method for Quantitation of Domperidone in Chinese Healthy Volunteers  

作　　者：WU Yi CHU Yang ZHANG Yun-hui WU Dan GU Jing-kai 

机构地区：[1]Research Center for Drug Metabolism, College of Life Science [2]College of Pharmacy, Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2007年第4期408-411,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.39930180).

摘　　要：A rapid, sensitive, and accurate method based on LC/MS/MS was developed and validated for the determination of domperidone in human plasma. Domperidone and internal standard, tramadol, were extracted from plasma with di- ethyl ether-dichloromethane （60： 40, volume ratio） and separated by reversed-phase HPLC with methanol-water-am- monia solution（80： 20： 0.2, volume ratio） as the mobile phase. Detection was carried out v/a multiple-reaction moni-toting（MRM） on a Q-trap^TM LC/MS/MS system（Q-trap^TM）. The assay result was linear over a concentration range of 0.1-30 ng/mL with a limit of quantitation （LOQ） of 0.1 ng/mL. The inter-and inn＇a-day precision levels were within 7.52% and 12.9%, respectively, whereas the accuracy was within a range of 87.3%-114%. This method has been successfully applied to evaluate the pharmcokinetics of domperidone in Chinese healthy volunteers given an oral dose of 10 mg.A rapid, sensitive, and accurate method based on LC/MS/MS was developed and validated for the determination of domperidone in human plasma. Domperidone and internal standard, tramadol, were extracted from plasma with di- ethyl ether-dichloromethane （60： 40, volume ratio） and separated by reversed-phase HPLC with methanol-water-am- monia solution（80： 20： 0.2, volume ratio） as the mobile phase. Detection was carried out v/a multiple-reaction moni-toting（MRM） on a Q-trap^TM LC/MS/MS system（Q-trap^TM）. The assay result was linear over a concentration range of 0.1-30 ng/mL with a limit of quantitation （LOQ） of 0.1 ng/mL. The inter-and inn＇a-day precision levels were within 7.52% and 12.9%, respectively, whereas the accuracy was within a range of 87.3%-114%. This method has been successfully applied to evaluate the pharmcokinetics of domperidone in Chinese healthy volunteers given an oral dose of 10 mg.

关 键 词：DOMPERIDONE LC/MS/MS Pharmacokinetics 

分 类 号：R969.1[医药卫生—药理学]