精索静脉曲张大鼠附睾功能变化的观察  

Change of Epididymis Function in the Experimental Varicocele Rat

作　　者：王海峰[1] 史本康[1] 张克勤[1] 李永智[1] 朱耀丰[1] 王海新[1] 

机构地区：[1]山东大学齐鲁医院泌尿外科,山东250012

出　　处：《生殖与避孕》2007年第6期374-377,共4页Reproduction and Contraception

基　　金：山东省人口和计划生育委员会基金(编号:2005-12)

摘　　要：目的:探讨实验性精索静脉曲张导致大鼠附睾功能降低的病理生理机制。方法:雄性Wister大鼠建立左侧精索静脉曲张模型,为实验组(n=11)并以假手术为对照组(n=9),免疫组化法检测附睾上皮p53的表达,DTNB法检测附睾中肉毒碱的含量,以5-甲基苯二酚法检测附睾中唾液酸的含量。结果:大鼠精索静脉曲张模型建立后的d28,实验组左侧附睾上皮p53表达显著高于对照组(P<0.05);实验组附睾中唾液酸、肉毒碱含量显著低于对照组(P<0.05)。结论:p53在精索静脉曲张后附睾功能降低的过程中起重要作用。Objective： To investigate the effect of p53 in the pathophysiology of varicocele. Methods： Experimental left varicocele models of 20 adult male Wister rats were obtained by partial ligation of the left renal vein, including control group （n=9） and experimental group （n=1 1）. The epididymis were collected 28 d after the surgery for detecting p53 expression with immunohistochemical method, the contents of sialic acid by spectrophotometry, and the carnitine content with DTNB. Results： The p53 expression of epididymis epithelium was significantly higher （P〈0.025）, and the content of sialic acid and carnitine in the epididymis were significantly lower （P〈0.05） in experimental group than in control group 28 d after the establishment of the left varicocele models. Conclusion： p53 plays an important role in the reduction of epididymis function in the experimental varicocele rats.

关 键 词：精索静脉曲张(VC) p53 唾液酸 肉毒碱 大鼠 附睾 

分 类 号：R697.24[医药卫生—泌尿科学] R363[医药卫生—外科学]