氧化苦参碱对兔油酸型急性肺损伤的保护作用研究  

Protective effects of oxymatrine on acute lung injury induced by oleic acid in rabbits

作　　者：陆晶[1] 张林丽[1] 余书勤[1] 吕伟[1] 蒋叶[1] 绪广林[1] 

机构地区：[1]南京师范大学生命科学学院新药研究中心江苏省医学分子生物学重点实验室,南京210097

出　　处：《中国新药杂志》2007年第14期1090-1094,共5页Chinese Journal of New Drugs

基　　金：江苏省高校自然科学重大基础研究项目资助(06KJA31024)

摘　　要：目的:研究氧化苦参碱对急性肺损伤的保护作用并探讨其机制。方法:采用静脉注射油酸的方法复制家兔急性肺损伤模型。动物随机分为6组:正常组、模型组、地塞米松组和氧化苦参碱组(20,40,80 mg.kg-1)。实验期间记录动物呼吸频率,不同时间点行血气分析,并与注射油酸后3 h时收集支气管肺泡灌洗液(BALF)测定白细胞介素-8(IL-8)和细胞间黏附分子-1(ICAM-1),实验结束测定肺干、湿重,计算湿/干重比,并行肺组织的病理学检查。结果:氧化苦参碱可明显减轻油酸模型组兔肺组织病理学改变,降低肺湿/干重比,升高PaO2/F iO2的比值,降低BALF中IL-8和ICAM-1的含量。结论:OMT能有效地保护油酸致家兔急性肺损伤,可能通过抑制细胞因子的过度生成从而达到肺保护作用。Objective： To investigate the effect of oxymatrine on acute lung injury（ ALI） induced by oleic acid in rabbits. Methods： The rabbits were randomly divided into 6 groups： normal group,oleic acid group, dexamethasone group, oxymatrine groups （20,40 and 80 mg·kg^-1）. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis at 30,60, and 120, 180 min after oleic acid injection. At the end of experiment, the concentrations of interleukin-8 （ IL-8 ） and intercellular adhesion molecule-1 （ ICAM-1 ） in bronchoalveolar lavage fluid （BALF） were measured and pathologic changes of lung tissues were observed under light microscopy. Results： Continuous infusion of oxymatrine significantly alleviated oleic acid-induced lung injury accompanied by improvement of pathological changes, reduction of wet-to-dry weight ratio, increases in the ratio of partial pressure of oxygen in artery （ PaO2 ） and fraction of inspired oxygen （ FiO2 ）, and decreases in IL-8 and ICAM-1 level in BALF. Conclusion： Oxymatrine has a beneficial effect on acute lung injury induced by oleic acid in rabbits by inhibition of inflammatory cytokine production.

关 键 词：氧化苦参碱 急性肺损伤 血气分析 白细胞介素-8(IL-8) 细胞间黏附分子-1(ICAM-1) 

分 类 号：R974[医药卫生—药品] R563[医药卫生—药学]