黄芩苷提取与纯化工艺的比较研究  

Comparative studies on the extraction and purification of baicalin

作　　者：王轶晶[1] 于洪儒[1] 王洪新[1] 杨菁[1] 

机构地区：[1]锦州医学院生物技术研究所,锦州121001

出　　处：《中国新药杂志》2007年第14期1101-1104,共4页Chinese Journal of New Drugs

摘　　要：目的:优化黄芩苷提取分离工艺。方法:采用正交设计,利用高效液相色谱法(High performance Liquid Chromatography,HPLC)检测生药粒度、不同提取纯化方法对黄芩苷纯度及得率的影响,优选提取纯化方案。以优选方案为实验组,常规煎煮法为对照组,进行平行实验。结果:20目生药粒度,微波提取6 min,超滤柱过滤后酸沉为优选的提取方案,黄芩苷得率为5.77 g%,纯度为89.98%。常规煎煮法黄芩苷得率为4.55 g%,纯度为77.77%。平行实验结果表明,优选方案在黄芩苷纯度及得率上都要明显高于常规煎煮法(P<0.05)。结论:微波提取、超滤柱过滤后酸沉的提取方法能够改善黄芩苷的提取率和纯度。Objective：To optimize the extraction and purification methods of baicalin from radix scutellariae. Methods：As compared to the conventional decoction method, orthogonal analysis was applied to new extraction and purification methods of baicalin by comparing the purity and yield rates of baicalin. A high performance liquid chromatography assay was used for measurements. Results：After a 20- mesh in granular particle size of radix scutellariae in water solution was treated with microwave extraction for 6 min, filtered by the ultra-filtration column and precipitated by acid, the yield rate of baicalin was 5.77% and purity of radix scutellariae was 89.98%. In contrast, the yield rates and purity were 4.55% and 77.77% obtained by conventional methods, exhibiting evident improvements on the extraction and purification methods （ P 〈 0.05 ）. Conclusions ： The microwave treatment and uhra-filtration followed by acid precipitation methods could improve the extraction and purification of baicalin from radix scutellariae.

关 键 词：正交试验 黄芩苷 提取分离 工艺优化 

分 类 号：R284.2[医药卫生—中药学]