青蒿琥酯对大鼠角膜新生血管的影响  

Effect of artesunate on rat corneal neovascularization

作　　者：孙京华[1] 王慧[1] 张煜昭[1] 刘常明[1] 王净信[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,湖北武汉430030

出　　处：《眼外伤职业眼病杂志》2007年第3期172-175,共4页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的研究青蒿琥酯(artesunate)对大鼠角膜新生血管形成的作用。方法采用碱烧伤诱导大鼠角膜新生血管模型,将65只SD大鼠随机分成正常对照组5只(5眼)、角膜新生血管对照组30只(30眼)、青蒿琥酯球结膜下注射组30只(30眼),比较第14d角膜新生血管生长的长度和面积,并分别在烧伤后第1、3、5、7、10、14 d用免疫组织化学染色的方法检测血管内皮生长因子(VEGF)、核因子κB(NF-κB)的表达。结果青蒿琥酯球结膜下注射显著抑制了角膜新生血管的生长,免疫组织化学染色VEGF、NF-κB在正常角膜上皮基底部有弱表达,在新生血管对照组有明显增强,青蒿琥酯球结膜下注射组表达明显减弱。结论青蒿琥酯球结膜下注射可以有效抑制角膜新生血管的生长,其治疗机制可能是抑制VEGF、NF-κB的表达。Objective To investigate the effects and mechanisms of artesunate on rat corneal neovascularization （CNV）. Methods Corneal neovascularization was induced by alkali burns on 60 adult male Sprague-Dawley rats. The experimental rats was randomly divided into CNV control group and artesunate group （treated by subconjunctival injection）, and another 5 adult male Sprague - Dawley rats were worked in normal control group. Digital slit lamp image system was used to evaluate neovascularization at the 14th day, and the length and area of CNV were measured in each group. The protein level of VEGF and NF-κB at 1st, 3rd, 5th, 7th, 10th, 14th days was studied by immunohistochemistry, and the results were analyzed by picture quantitative analysis techniques. Results Mean length of neovascularization at the 14th day was 2.54 ±0. 15mm for CNV control group and 1.02 ±0. 16mm for artestunate group. Statistical analysis showed significant differences between CNV control group and artesunate group （t = -15.2, P 〈 0. 01 ）. Mean area of neovascularization at the 14th day was 27. 552 ± 0. 490 mm^2 for CNV control group and 15. 892 ± 2.063mm^2 for artesunate group, Differences between them were statistically significant （t = 12. 299,P 〈 0.01 ）. The protein level of VEGF and NF - κB in the cornea was markedly increased in CNV control group in comparison with normal control group and suppressed significantly in artesunate group. Conclusions Artesunate can inhibit corneal neovasculars by suppressing the expression of VEGF and NF-κB.

关 键 词：青蒿琥酯 角膜新生血管 烧伤 化学 血管内皮生长因子 核因子ΚB 

分 类 号：R285.5[医药卫生—中药学]