条斑紫菜多糖抗疲劳生物活性研究  

Study on Anti-fatigue Bioactivity of Polysaccharide from Porphyra yezoensis

作　　者：顾佳雯[1] 张陆曦[1] 郭婷婷[1] 何培民[1] 

机构地区：[1]上海水产大学生命学院中心实验室,上海200090

出　　处：《生物技术通报》2007年第4期120-124,131,共6页Biotechnology Bulletin

基　　金：上海市科委重大攻关项目(054319936);上海市科委浦江人才计划项目(05PJ14086);上海市教委优势(重点)学科资助项目(Y1101)

摘　　要：主要应用活体动物实验技术,研究了纯化产物紫菜多糖的抗疲劳作用及量效关系。通过紫菜多糖PY-G1不同剂量对小鼠游泳时间、乳酸脱氢酶活性、肌糖原和肝糖原含量等指标的检测,分析了紫菜多糖提高小鼠抗疲劳生物活性功能及其量效关系。实验结果表明,紫菜多糖可显著延长小鼠游泳时间,最高可以提高37%;并且可以使小鼠在游泳前后乳酸脱氢酶含量分别增加35%和37.5%(P<0.05);增加小鼠肌糖原储备量和肝糖原储备量,其肌糖原储备量和肝糖原储备量最高可以分别增加85.6%和86%。实验结果表明,条斑紫菜多糖具有明显提高小鼠抗疲劳生物学活性。In this paper,anti-fatigue effects of Porphyra yezoensis polysaccharide and their dose-effect relationship was studied with live-animal experiment.By measureing the effect of different dosage of Porphyra polysacccharide on mice swimming time,lactate dehydrogenase （LDH）activity of mice before and after swimming,and the content of muscle glycogen and hepatic glycogen of mice,for identifying Porphyra polysaccharide could increase anti-fatigue bioactivity of mices.The experiment results showed that PY polysaccharide could prolong mice swimming time signifcandy,increasing as high as 37% ,and it could enhance the reserves amount of muscle and hepatic glycogen,by 185.6% and 186% respectively;and the polysaccharide also could increase LDH activity by 135% and 137.5% before and afrer swimming respectively.It indicated that polysaccharide from Porphyra yezoensis had the antifatigue bioactivity.

关 键 词：条斑紫菜 多糖 抗疲劳 糖原 乳酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]