甲基叔丁基醚对大鼠小脑γ-氨基丁酸影响  

Effect of methyl tertiary butyl ether on γ-amino-butyric acid in cerebral of rat

作　　者：李素云[1] 贺性鹏[2] 邓暑芳[2] 万炜[1] 范松青[1] 

机构地区：[1]南华大学医学院解剖学教研室,湖南衡阳421001 [2]南华大学公共卫生学院劳动卫生与环境卫生教研室,湖南衡阳421001

出　　处：《中国公共卫生》2007年第8期968-969,共2页Chinese Journal of Public Health

基　　金：湖南省卫生厅科研项目基金(B2005090)

摘　　要：目的研究甲基叔丁基醚（Methyl tertiary butyl ether，MTBE）对大鼠小脑皮质神经递质γ-氨基丁酸（γ-GABA）的影响，探讨MTBE对中枢神经系统的急性系作用机制。方法54只健康成年SD大鼠，体重180～220g，随机分为9组，腹腔注射染毒，染毒剂量分别为0，700，1050，1400mg／（kg·bw）。观察各染毒剂量及染毒后0．5，1，2，4，8h时MTBE对大鼠小皮质神经递质GABA的影响。结果MTBE对小脑皮质神经递质GABA的作用其阳性产物相对灰度值结果显示，不同剂量[700，1050，1400mg／（kg·bw）]MTBE染毒后小脑皮质神经递质GABA相对灰度值随着染毒剂量增加而增加，存在剂量-效应关系；MTBE对小脑皮质GABA的影响在染毒后0．5h时GABA表达开始增加，1．0h达到高峰，2．0h时开始减弱，8．0h时GABA表达恢复到正常水平。结论MTBE对中枢神经系统急性毒作用与神经递质GABA含量的改变有关。Objective To explore the acute toxic mechanism of methyl tertiary butyl ether （MTBE）, in the cerebellar cortex by detecting the change of the neurotransmitter γ- amino - butyric acid （ GABA）. Methods 54 adult SD rats were randomly divided into control and eight experiment groups. The MTBE exposed doses were 700, 1 050, 1 400mg/（ kg· bw） and the control group was used normal saline 1 400 mg/（kg· bw）respectively injected by abdominal cavity. Then the relative grey value of GABA expression was mensurated after exposed MTBE in 0.5, 1.0, 2.0, 4.0, 8.0 h in cerebellar cortex. Results The expression of the GABA in cerebellar cortex was inceased when exposed MTBE [700, 1 050, 1 400 mg/（kg·bw）]. The expression of the GABA in cerebellar cortex was changed in the different time （0.5, 1.0, 2.0, 4.0, 8.0 h）after being exposed to MTBE （ 1 050 mg/（ kg· bw） ）. The expression of the GABA began to increase in 0.5 h, reached peak in 1.0 h, began to reduce in 2.0 h, and was recovered to normal level in 8.0 h. Conclusion The acute toxic effect of MTBE in central nervous system is relevant to the neurotransmitter GABA.

关 键 词：甲基叔丁基醚 小脑皮质 Γ-氨基丁酸 

分 类 号：R994.6[医药卫生—毒理学]