胞二磷胆碱对神经细胞凋亡保护作用  

Neuroprotective effects of citicoline on apoptosis of neurons induced by 6-hydroxydopamine

作　　者：张卫民[1] 姜晓燕[2] 魏丽晶[3] 李艳博[4] 郭伟[4] 龚守良[4] 

机构地区：[1]吉林省卫生厅卫生监督所,长春130062 [2]长春市卫生局卫生监督所 [3]吉林大学第一临床医院检验科 [4]吉林大学公共卫生学院卫生部放射生物学重点实验室

出　　处：《中国公共卫生》2007年第8期1006-1007,共2页Chinese Journal of Public Health

摘　　要：目的探讨胞二磷胆碱对6-羟基多巴胺所致的神经细胞凋亡的保护作用机制。方法采用流式细胞仪检测神经细胞的凋亡百分比及Bax、Bcl-2蛋白表达。结果将1,0.1,0.01和0.001mmol/L胞二磷胆碱+6-羟基多巴胺组,与6-羟基多巴胺组比较,凋亡百分比明显降低(P<0.01);各组Bax蛋白的表达率明显下降,0.1mmol/L胞二磷胆碱组明显低于对照组;1,0.1和0.01mmol/L胞二磷胆碱组Bcl-2表达率显著升高;Bax/Bcl-2比值下降,0.1mmol/L胞二磷胆碱组Bax/Bcl-2比值低于对照组(P<0.01)。结论胞二磷胆碱通过减少Bax和增加Bcl-2蛋白的表达,使Bax/Bcl-2比值下降、细胞凋亡减少,发挥其神经保护作用。Objective To study the neuroprotective effects of citicoline on apoptosis of neurons induced by 6-hydroxy- dopamine in vitro and its mechanism. Methods The percentage of apoptosis and the expressions of Bax and Bcl-2 proteins in primary cultured neurons were detected by flow cytometer. Results As compared with 6-hydroxydopamine group, 1, 0.1, 0.01 and 0. 001 mmol/L citicoline group the apoptosis of neurons by 6-hydroxydopamine - induced were decreased significantly, P 〈 0.01;The expressions of Bax proteins were decreased and Bcl-2 proteins were increased, Bax/Bcl-2 ratio was lower than that of 6-hydroxydoparnine group, P 〈 0.01. Conclusion The neurotoxic effect of 6 - hydroxydopamine can be reduced by citicoline to decreese the percentage of neurons apoptosis. By downregulating the expressions of Bax proteins and upregulating expressions of Bcl-2 proteins, the apoptosis of neurons can be decreased and neurons were protected by citicoline.

关 键 词：胞二磷胆碱 6-羟基多巴胺 凋亡 凋亡蛋白 帕金森病 神经保护 

分 类 号：R113[医药卫生—公共卫生与预防医学]