白木香核型与Giemsa C-带带型研究  

Study on Karyotype and Giemsa C-Banding of Aquilaria sinensis

作　　者：申彦晶[1] 赵树进[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510641

出　　处：《中药材》2007年第7期762-765,共4页Journal of Chinese Medicinal Materials

基　　金：广东省科技厅项目(19981212)

摘　　要：目的:研究白木香染色体的核型与C-带带型,为该种鉴定、起源、良种培育等的研究提供细胞学资料。方法:核型分析采用常规压片法,C-带带型用改良BSG法。结果:白木香体细胞染色体数目2n=16,核型公式为K(2n)=2x=16=6m+6sm+4st,染色体相对长度组成2n=16=4L+4M2+6M1+2S,属于"2B"型。白木香8对染色体共有34条C-带带纹,C-带带型公式为2n=2x=16=4C+2I+2T+2CI++2CI+T+2CI+T++2I+T+。结论:白木香染色体的不对称性较高,处于较进化地位,为进一步研究提供参考。Objective： To study Aquilaria sinensis （Lour.） Spreng chromosome karyotype and C-banding. Method： Sections for karyotype and BSG method for C-banding. Results： The somatic chromosome number was 2n = 16, karyotype formula was K （2n） = 2x = 16 = 6m ＋ 6sm ＋ 4st, based on Levan＇s publication in 1964. According to the method of Kuo, the chromosome based on the relative length was 2n = 16 =4L ＋4M2 ＋6MI ＋ 2S, which belonged to ＂2B＂. 8 pairs chromosomes had 34 C-bands and the C-banding patter was 2n = 2x = 16 =4C ＋ 2I ＋ 2T ＋ 2CI＋ ＋ 2CI＋ T ＋ 2CI＋ T^＋ ＋ 2I＋ T^＋. Conclusion ： The data of karyotype and C-banding indicated A. sinensis chromosome had a relatively high asymmetry and was in the advanced stage of evolution, which offered the evidence for further genetic analysis.

关 键 词：白木香 核型分析 C-带 

分 类 号：R282.2[医药卫生—中药学]