丹参酮ⅡA磺酸钠对兔髂动脉球囊损伤后血管内膜增殖的抑制  

The Inhibitive Effect of Sodium Tanshinone Ⅱ A Sulfonic Acid on Intimal Hyperplasia in Rabbit Iliac Artery Balloon Injury Model

作　　者：陈玉成[1] 陈怀生[1] 龙波[1] 梁玉佳[1] 曾智[1] 

机构地区：[1]四川大学华西医院心内科,四川成都610041

出　　处：《中药材》2007年第7期811-815,共5页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅应用基础研究项目(04JY029-079-1)

摘　　要：目的：观察丹参酮ⅡA磺酸钠对兔髂动脉球囊损伤后血管新生内膜增殖的影响和相关机制。方法：取纯种雄性新西兰大白兔30只，采用球囊导管扩张法制作左髂动脉内膜球囊损伤模型，术后按体重配对分配到治疗组和对照组。治疗组丹参酮ⅡA磺酸钠7．5～9mg／只／天，稀释为4ml溶液静脉注射，疗程6天；对照组采用等量生理盐水静脉注射。术后7天、14天和28天处死实验兔，并取出左侧髂动脉，制作石蜡切片，分别行HE染色、凋亡的TUNEL病理分析。结果：（1）HE染色形态学分析显示：术后14天治疗组中膜、内膜面积比7天有所增加，但小于对照组（P值分别为0．003和〈0．001）；术后28天治疗组中膜、内膜面积较术后14天减少，而对照组仍保持增加趋势，两组中膜、内膜面积均有明显差异（P值均〈0．001）。（2）细胞凋亡TUNEL分析：术后28天治疗组和对照组中膜、内膜凋亡细胞数均达到最大；术后7、28天治疗组与对照组中膜、内膜凋亡细胞数无明显差异，而术后14天两组中膜、内膜凋亡细胞数差异均较明显（P值分别为0．031和0．029）。结论：静脉注射丹参酮ⅡA磺酸钠有抑制动脉损伤后内膜增殖的作用，这一作用可能与诱导细胞凋亡等机制有关，但其临床实际运用有待进一步研究。Objective ： To study the preventive effect of Sodium Tanshinone Ⅱ A sulfonic acid on intimal hyperplasia in rabbit iliac artery balloon injury model and explore the possible mechanism. Methods： Thirty male pure breed New Zealand white rabbits were undertaken experimental balloon injury, in left iliac artery. Then the rabbits were assigned into treatment group（ n = 15） and control group （ n = 15）, paired with weights. Sodium Tanshinone Ⅱ A sulfonic acid had been injected intravenously with 7.5 - 9 mg/day for 6 days in treatment group. Saline of equivalence was given in contol group. The balloon injured arteries were harvested in the 7th, 14th, and 28th days after balloon injury, and Paraffin sections were made. At last, HE staining, apoptosis TUNEL assay were undertaken. Results： （ 1 ）HE staining analysis： Media and intimal areas in treatment group at 14th day post-operation were larger than that in the 7th day（P =0. 003 and 〈0. 001, respectively）. Media and intimal areas in treatment group decreased at the 28th day post-operation, while increased in control. Both media and intimal areas were significantly different（ P 〈 0. 001 respectively. （2）Tunel analysis discovered that, apoptosis reached peak in both treatment and control groups at the 28th post-operation. Differences of apoptosis cells counts in media and intimal between treatment and control groups were non-significant at the 7th, and 28th days, while differences at the 14th day were significant（ p = 0. 031 and 0. 029 respectively）. Apoptosis cells counting in treatment group at the 14th day increased more dramatically than that in the control. Conclusion ： Intrvenous Sodium Tanshinone Ⅱ A sulfonic acid inhibites intimal proliferation after arterial balloon injury in rabbits. The effect can be partially explained by the induction of apoptosis in injured artery. Clinical effect of tanshinone Ⅱ A still needs further evaluation. Sodium TA- Ⅱ A sulfonic acid may be of potential therapeutic value in the preventi

关 键 词：髂动脉 球囊损伤 丹参酮ⅡA磺酸钠 新生内膜 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]