多房棘球绦虫重组BCG-Em Ⅱ/3疫苗对小鼠脾细胞凋亡的影响  

Effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with Echinococcus multilocularis

作　　者：杨梅[1] 李文桂[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《重庆医科大学学报》2007年第8期796-798,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金(30671835;30500423和30200239);教育部重点项目(205131);重庆市教委科研基金(KJ050313);重庆市科委科研基金(03-43-8);重庆市卫生局科研基金(03-2-099)

摘　　要：目的:检测多房棘球绦虫重组BCG-Em Ⅱ/3疫苗对原头蚴攻击小鼠脾细胞凋亡的影响。方法:用重组BCG-Em Ⅱ/3疫苗分别通过皮下注射和鼻腔粘膜接种免疫BALB/C小鼠8周后,用50个多房棘球绦虫原头蚴腹腔注射进行攻击,攻击感染后18周剖杀小鼠,分离脾细胞,用ConA刺激培养16～18h,然后用Annexin V-FITC染色法检测脾细胞凋亡。结果:小鼠脾细胞原液组和ConA刺激组细胞凋亡发生率均显著低于PBS对照组(P<0.05或P<0.01);每个ConA刺激组脾细胞凋亡发生率显著高于相应的原液组(P<0.05或P<0.01);鼻腔粘膜接种组脾细胞凋亡率显著低于皮下注射组(P<0.01)。结论:多房棘球绦虫重组BCG-EmⅡ/3疫苗可抑制感染小鼠脾细胞发生凋亡,增加CD4+T细胞亚群的数量,加强宿主抗多房棘球绦虫感染的保护性免疫反应。Objective：To detect the effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with protoscoleces of Echinococcus muhilocularis. Methods：BALB/C mice were immunized with rBCG-Em Ⅱ/3 vaccine by subcutaneous injection or intranasal inoculation.After 8 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection. On the 18th week after infection,mice were killed to separate splenocytes and stimulated with ConA for 16-18h,then apoptosis of splenocytes was detected by Annexin V-FITC staining methods. Results：Apoptotic rate of splenocytes in all groups with or without ConA stimulation was obviously lower than that of PBS group （P〈0.05 or P〈0.01 ）;Apoptotic rate of splenocytes in each group with ConA stimulation was greatly higher than that in the group without ConA stimulation （P〈0.05 or P〈0.01 ）.Apoptotic rate in intranasal inoculation group was also lower than that in subcutaneous injection group （P〈0.01）. Conc/usions：rBCG-Em Ⅱ /3 vaccine may inhibit apoptosis of splenocytes in mice challenged with Echinococcus muhilocularis,increase the number of CD4^＋ T lymphocyte subsets,and enhance host＇s protection against the challenge with protoscoleces of Echinococcus multilocularis.

关 键 词：多房棘球绦虫 重组BCG-EmⅡ/3疫苗 脾细胞 凋亡 

分 类 号：R383.3[医药卫生—医学寄生虫学]