rhVEGF对大鼠成骨细胞增殖及功能的影响  

Effects of recombinant human vascular endothelial growth factor on proliferation and differentiation of rat osteoblasts

作　　者：兰泽栋[1] 吴纪程[2] 张端强[2] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院,510280 [2]福建医科大学口腔医学院

出　　处：《实用口腔医学杂志》2007年第4期550-553,共4页Journal of Practical Stomatology

摘　　要：目的:研究rhVEGF对成骨细胞增殖和分化能力的影响。方法:采用细胞培养技术,应用光镜、MTT法及PNPP偶氮法,分别从细胞形态、细胞增殖及分化等特性,分析不同浓度的rhVEGF对成骨细胞增殖和碱性磷酸酶活性的影响。结果:rhVEGF能促进成骨细胞的增殖和分化,其中,在加入3.125ng/ml浓度的rhVEGF并于第3天时,成骨细胞增殖最显著;而12.5ng/ml组对碱性磷酸酶活性的影响最明显。结论:一定剂量的rhVEGF可明显促进成骨细胞的增殖和分化。Objective：To study the effects of recombinant human vascular endothelial growth factor on proliferation and differentiation of rat osteoblasts. Methods： Cell culturing, light microscop, MTT assay and PNPP assay were performed to observe the effects of rhVEGF on proliferation and differentiation of the cells. Results： rhVEGF could stimulate osteoblasts proliferation and differentiation. The maximal effect on proliferation was observed at a concentration of 3. 125 ng/ml on the third day. ALP activities in osteoblasts were increased most significantly at a concentration of 12.5 ng/ml. Conclusion： rhVEGF may not only induce angiogenesis to facilitate bone formation, but also stimulate this process in a direct way by inducing proliferation and differentiation of osteohlasts.

关 键 词：重组人血管内皮生长因子 成骨细胞 增殖 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]