复合人工骨修复犬股骨头缺损坏死中再血管化与钙含量的关系  

Correlation between revascularization effect and calcium content of a kind of man-made compound bone on the femoral head defects and necrosis of canines

作　　者：刘钢[1] 彭吾训[1] 邓进[1] 龚跃昆[2] 李世和[2] 

机构地区：[1]贵阳医学院附属医院急诊外科,贵州贵阳550004 [2]昆明医学院第一附属医院骨科,云南昆明650032

出　　处：《中国矫形外科杂志》2007年第15期1172-1174,共3页Orthopedic Journal of China

摘　　要：[目的]观察骨形成蛋白(bone morphogenetic protein,BMP)、碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)与异体脱抗原松质骨(allogeneic antigen-extracted cancellous bone,AACB)复合后修复股骨头坏死(femoral head necrosis,FHN)病灶清除区缺损,评价bFGF对FHN的再血管化作用及其与钙含量的关系。[方法]取18只杂种犬共36侧股骨头,建立液氮冷冻诱导性犬股骨头缺损坏死模型,随机分为A、B、C3组,每组12侧。A组为空白对照,B组植入AACB/BMP,C组植入AACB/BMP/bFGF。每个股骨头内植入AACB约0.33g,BMP约12.5mg,bFGF约2000U。术后3、6和12周分批处死动物,每组每次处死2只。行组织学观察,免疫组织化学染色,进行血管计数和血管面积图像分析、钙含量测定、并分析再血管化作用与钙含量间的关系。[结果]组织学观察,12周时A组以纤维结缔组织为主,B组多量新生骨组织形成,密度不均,C组股骨头骨缺损完全修复。血管计数和血管面积,C组术后3周移植物孔隙内大量血管增生,6和12周时血管数量和血管面积进一步增加,各时间点都大于A、B组,具有统计学意义(P<0.05)。钙含量,C组术后3周钙含量小于B组,12周时大于B组(P<0.05)。再血管化作用与钙含量的关系,C组与B组比较,术后3周时再血管化与钙含量呈负相关,12周时呈正相关。[结论]AACB是生长因子的良好吸附载体,适宜新生血管长入。吸附有bFGF及BMP的AACB具有较强的再血管化能力,bFGF促进植骨材料吸收,加速新骨形成。这一疗法有望成为FHN治疗的一种手段。[ Objective] To observe the effect of composite （AACB/BMP/bFGF） of allogeneic antigen-extracted cancellous bone （ AACB）, bone morphogenetic protein （BMP） and basic fibroblast growth factor （bFGF） on the repair of defective and necrotic areas of the femoral head, and evaluate the revaseularization the femoral head defect and necrosis model using liquid nitrogen, and randomly divided into 3 groups with 12 femoral heads in each group, which were implanted with nothing （group A）, AACB/BMP （group B） and AACB/BMP/bFGF （group C） respectively. Every femoral head was implanted with about AACB 0. 33 g, BMP 12. 5 mg and bFGF 2000 U. Two canines were sacrificed respectively at 3^th, 6^th, and 12^th weeks after operation in every group. Histologically, immunohitochemical vessel staining, vessel count and vessel surface area image analysis, calcium content and the correlation between revascularization effect and calcium content were investigated in the femoral heads after the operation. [ Result ] Histology, the detects were still infilled by fibrillar tissue in group, A; there were a great deal of new bone formed in group B; and the detects were repaired completely in group C 12 weeks after operation. Measurement of vessel count and vessel surface area showed a large quantity of new vessel proliferated around the infilling material in group C on the 3rd week. The vessel count and vessel surface area continuously increased on the 6th week and 12th week. They were more than those of group B and group A at every definite time point （ P 〈 0. 05 ）. Calcium content in group C was less than that of group B on the 3rd, and more than that of group B on the 12th week （P 〈 0. 05 ）. The correlation between revascularization effect and calcium content showed negative correlation on the 3rd week and positive correlation on the 12th week when that of group C was compared with that of group B （ P 〈 0. 05 ）.[ Conclusion] AACB, a kind of fine carrier of growth factors, is suitable for new vessel gr

关 键 词：股骨头坏死 骨形成蛋白 碱性成纤维细胞生长因子 异体骨 

分 类 号：R318.1[医药卫生—生物医学工程]