长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立  

A Study on Induction of Emdryogenic Callus and Plant Regeneration from Mature Embryos of Elytrigia hybrid(E. elongata×E. intermedia)

作　　者：鲍芫[1] 米福贵[1] 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010018

出　　处：《草地学报》2007年第4期348-351,共4页Acta Agrestia Sinica

基　　金：国家转基因植物与产业化研究重大专项项目(J2002-B-008)

摘　　要：以长穗偃麦草(Elytrigiaelongata)和中间偃麦草杂交种(Elytrigia hybrid;E. elongata×E. intermedia)的成熟胚为外植体,进行组培再生体系的研究。结果表明:诱导愈伤组织的适宜培养基为MS+CH(500 mg/L)+2,4-D(1.0 mg/L)+6-BA(0.2 mg/L),其诱导率为73%,适宜分化培养基为MS+6-BA(4.0 mg/L)+NAA(0.5 mg/L),分化率最高,为37.8%。本研究建立了一套有效的以成熟胚为外植体偃麦草杂种组织培养再生体系。The regeneration from mature embryos was studied using the mature embryos of Elytrigia hybrid （E. elongata × E. intermedia） as explants. Results indicate that the frequency of callus induction reached 73% in the callus induction medium supplemented with 1.0 mg/L 2,4-D and 0.2 mg/L 6-BA. During subcultures, rapid growth of callus and embryogenesis were maintained in the callus subculture medium plus 1.0 mg/L 2,4-D and 0.5 mg/L 6-BA. After callus subcultures, the rate of shoot regeneration from callus were 37.80/oo in the differentiation medium supplemented with 4.0 mg/L6-BA and 0.5mg/L NAA. An effective system of mature embryos regeneration for Elytrigia hybrid （E. elongata × E. intermedia） was developed.

关 键 词：长穗偃麦革 中间偃麦草 杂交种 成熟胚 愈伤组织 再生植株 

分 类 号：S812[农业科学—草业科学] Q943.1[农业科学—畜牧学]