HBV前C/C及其调控序列热点变异与肝炎病变程度的关系  

Correlation between hot spot mutation in HBV PreC/C, BCP and disease progress

作　　者：梁蔚芳[1] 何海棠[1] 刘志华[1] 骆抗先[1] 

机构地区：[1]南方医科大学南方医院感染内科,广东广州510515

出　　处：《第四军医大学学报》2007年第15期1405-1407,共3页Journal of the Fourth Military Medical University

摘　　要：目的:通过对慢性HBV患者中HBV preC/C基因及其调控序列的变异热点的分析,研究各区段变异热点与疾病病变程度的关系.方法:对42例慢性HBV患者中扩增的HBV DNA各3个克隆进行序列分析.结果:42例不同病变程度的慢性HBV中,T1762/A1764双突变在轻度慢性肝炎中发生变异5例(38.5%),中度慢性肝炎发生变异4例(30.8%),重度慢性肝炎发生变异11例(68.7%),T1762/A1764双变异在三组患者中的分布无差异(P>0.05).前CA1896变异在轻度慢性肝炎中发生率为30.8%,在中度慢性肝炎中发生率为46.2%,在重度慢性肝炎中的发生率为50.0%,A1896变异在三组患者中分布的差异无统计学意义(P>0.05).HBV/C区的变异热点中,轻度慢性肝炎患者中发生氨基酸替代合计23个,中度慢性肝炎患者中发生氨基酸替代合计33个,重度慢性肝炎患者中发生氨基酸替代合计40个.在三组患者中C区氨基酸替代的分布有统计学差异(P<0.05),替代随病变程度的加重而增加.结论:慢性HBV患者的HBVC基因及其调控序列变异具有多样性和复杂性,与体内的免疫清除和病毒逃避免疫攻击相关,从而容易导致疾病的慢性化.AIM： To study the mutants of preC/C gene and the base core premotor （BCP） in 42 patients with different phases of chronic hepatitis B, so as to find out the relationship between hot spots in different regions and the disease progress. METHODS： The HBV DNA of BCP and PreC/C were amplified by PCR from serum samples of 42 patients with chronic hepatitis B, and the products were cloned into T vector and sequenced. RESULTS： Of the BCP double mutations, the T1762/A1764 mutants occurred in 5 patients （38.5%） in the mild hepatitis group, 4 patients （30. 8%） in the moderate hepatitis group and 11 patents （68.7%） in the severe hepatitis group. The T1762/A1764 double mutations were not significantly different among the three groups （P 〉 0.05）. A1896 mutation occurred in 30.8% of mild hepatitis group, in 46.2% of moderate hepatitis group and in 50.0% of severe hepatitis group. The A1896 mutation was not significantly different among the three groups （ P 〉 0.05 ）. Of the hot spots in the core region, there were 23 amino acids substituted in mild hepatitis group, 33 in moderate hepatitis group and 40 in severe hepatitis group. The amino substituted in the core region was significantly different among the three groups （ P 〈 0.05 ）, and the substitutes were increased along with the disease progress. CONCLUSION： The mutation in the preC/C and BCP gene is multiple and complex and associated with chronic hepatitis prograssion and immune clearance.

关 键 词：乙型肝炎病毒 慢性乙型肝炎 突变 

分 类 号：R373[医药卫生—病原生物学]