HPLC法测定复方鼻咽洗剂中绿原酸的含量  

Content determination of chlorogenic acid in compound nasopharyngeal lotion by HPLC

作　　者：黄小玲[1] 彭解人[2] 梁象逢[2] 卢日铭[3] 

机构地区：[1]深圳市慢性病防治院,广东深圳518019 [2]中山大学附属第二医院,广东广州510120 [3]深圳市中医院,广东深圳518033

出　　处：《中国医药导报》2007年第08Z期88-90,共3页China Medical Herald

基　　金：2003广东省中医药局立项课题(编号:103121)

摘　　要：目的：建立复方鼻咽洗剂中绿原酸含量的测定方法。方法：采用高效液相色谱法,色谱柱为DIKMA Diamonsil C18（150mm×4.6mm,5μm）,柱温40℃,流动相为乙腈-0.4%磷酸溶液（13∶87）,流速为1.0ml/min,检测波长为327nm。结果：绿原酸的回归方程为Y=2468778.0082X＋2128.1393,相关系数r=0.9996,线性范围0.04-0.40μg,平均回收率为99.75%,RSD=1.82%（n=9）。结论：本法简便、准确、重现性好、适用性强,可作为复方鼻咽洗剂的质量控制方法。Objective：To establish a method for the determination of chlorogenic acid in compound nasopharyngeal lotion. Methods： HPLC was used with chromatographic conditions including DIKMA Diamonsil C18 （150 mm×4.6 mm,5 μm）.The column temperature was 40℃ ,the mobile phase was acetonitrile-phosphoric acid （13：87）,the flow rate was 1.0 ml/min, and the detection wavelength was 327 nm. Results： The linear calibration curve （Y=2 468 778.008 2X＋2 128.139 3 ,r=0.999 6） was obtained in the concentration range of 0.04-0.40μg/ml,and the average recovery was 99.75% with RSD of 1.82%（ n= 9 ）. Conclusion： The method is simple and accurate with good reproducibility, suitable for quality control of compound nasopharyngeal lotion.

关 键 词：高效液相色谱法 复方鼻咽洗剂 绿原酸 含量测定 

分 类 号：R917[医药卫生—药物分析学]