小麦突变体D51抗秆锈性遗传分析及其抗性基因SSR标记  

Genetic Analysis and SSR Mapping of Stem Rust Resistance Gene from Wheat Mutant D51

作　　者：尹静[1] 王广金[2] 张宏纪[1] 孙岩[1] 刁艳玲[1] 黄景华[1] 郭强[1] 肖佳雷[1] 马凤鸣[3] 孙光祖[2] 

机构地区：[1]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院生物研究中心,黑龙江哈尔滨150086 [3]东北农业大学,黑龙江哈尔滨150030

出　　处：《作物学报》2007年第8期1262-1266,共5页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2002AA241011);黑龙江省杰出青年基金项目(2005-2007)

摘　　要：D51是优良品系龙6239经辐射诱变和组织培养相结合获得的高产优质抗秆锈突变体材料,对我国优势秆锈病21C3CPH、21C3CKH和21C3CTR小种均表现免疫。利用来自D51/龙6239、D51/中国春的2个F2群体和来自D51/龙6239的1个F2群体分别在苗期和成株期进行21C3CPH小种接种,抗病反应型鉴定表明,3个F2群体中抗感分离比例均为3∶1,说明D51抗秆锈性受一对显性基因控制,全生育期表达,部分D51/龙6239F2植株的F3株系的抗病鉴定进一步验证F2鉴定的可靠性。利用675对小麦SSR引物和185株D51/龙6239F2分离群体对SrD51基因进行标记定位,将SrD51定位在5D染色体的短臂上。其中SSR标记Xgwm190和Xwmc150与SrD51基因的遗传距离分别为18.58和21.33cM,并分别位于目的基因的两侧。由于已知的秆锈抗性基因仅有Sr30被定位在小麦5DL上,且Sr30不抗34C2MKK和34C2MFK,而突变体D51的原亲本龙6239不抗21C3CPH、21C3CKH和21C3CTR,却对34C2MKK和34C2MFK表现免疫抗性。因而推断此突变体的秆锈抗性基因可能是一个新基因,暂命名为SrD51。Stem rust caused by Puccinia graminis f. sp. tritici is one of the main diseases of wheat （ Triticum aestivum L. ） worldwide. Wheat mutant line D51, form a highly susceptive cultivar ‘16239＇ to the three races notated and cultured with immatttre embryo, shows resistance to prevailing races 21C3CPH, 21C3CKH, and 21C3CTR of P. graminis f. sp. tritici in China. In this paper, number of the resistance genes in mutant D51 and the expression stages were studied with inoculation identification and microsatellite （SSR） marker analysis. Two F1 populations from the crosses of 1351 ×L6239 （60 individuals ） and D51 × Chinese Spring （60 individuals）, their F2 populations （ 185 and 175 individuals respectively ） at seedling and one F2 populations derived from the cross of D51 ×L6239 （ 194 individuals） at adult stage were inoculated with pathogen race 21C3CPH to test their resistance. All F1 individuals of the two crosses were immune to stem rust at both seedling and adult stages. The response pattern of the three F2 populations showed that the R：S segregation ratio fit 3：1, suggesting the stem rust resistance of D51 controlled by a single dominant gene, and expressed in whole growth period. The identification to stem rust resistance by F3 progeny test confirmed the credibility of F2 population test. Segregating pepulatious and small population analyses were used to identify chromosomal region and molecular markers linked to the gene by SSR markers method. A total of 675 SSR markers and 185 individuals of D51×16239 F2 population were used to search genetically linked makers to the target gene. Using Mapmaker3.0 and Map-draw with Kosambi＇ s fimction and other options left at default values, molecular mapping revealed that the gene was located on the chromosome 5DS, and linked with and blanked by two SSR markers Xgwml90 and Xwmc150 at 18.58 and 21.33 cM, respectively. According to references reported, the only one stem rust resistant gene Sr30 is located on the wheat chromosome 5DL, and has n

关 键 词：小麦 秆锈病 抗性基因 SSR标记 

分 类 号：S512.1[农业科学—作物学]