南昌市德国小蠊抗药性及羧酸酯酶生化特性的相关性研究  

The insecticide resistant of the Blattella germanica in Nanchang and its relationship with the biochemical characterization of Carboxylesterase

作　　者：马红梅[1] 陶卉英[1] 陈海婴[1] 柳小青[1] 郭学俭[1] 燕念如[1] 曹文峰[1] 

机构地区：[1]南昌市疾病预防控制中心消毒与生物媒介防制所,南昌330006

出　　处：《中国媒介生物学及控制杂志》2007年第4期283-286,共4页Chinese Journal of Vector Biology and Control

摘　　要：目的研究南昌市野生品系德国小蠊对当地常用杀虫剂的抗药性水平,并对野生与敏感品系德国小蠊的羧酸酯酶(CarE)的差异进行比较研究。方法用药膜法测定KT50,参照Van Asperen的方法测定CarE的比活力、米氏常数(Km)及最大反应速度(Vmax)值。结果南昌市野生品系德国小蠊对敌敌畏、残杀威、溴氰菊酯、右旋反式丙炔菊酯、高效氯氰菊酯抗性系数分别为>122.3、1.1、1.4、5.3、4.9。野生品系与敏感品系德国小蠊CarE比活力分别为68.7567和56.5069mmol/(mgpro.30 min);Km分别为0.4171和0.4374 mmol/L;Vmax分别为0.8525和0.4075 nmol/(mgpro.min)。结论南昌市野生品系德国小蠊已对有机磷类、拟除虫菊酯类杀虫剂产生抗药性,这种抗药性的产生与CarE生化特性的改变密切相关。Objective To evaluate the resistance of Blattella germanica against common used insecticides in Nanchang, and to study the characteristic of carboxylesterase（CarE） in both field and susceptible population. Methods The surface contact method of WHO recommended for cockroach were used for B. germanica insecticides resistance test, and Van Asperen method were used for Care activity. Results The resistance indexes of dichlorvos, popoxur, dehamethrin, beta cypermethrin were 〉 122.3, 1.1, 1. 4, 5.3, 4.9 respectively in field population comparing to susceptible strain. The specific activities of the resistant and sensitive strains were 68. 7567 mmol/（mgpro·30 min）, 56. 5069 mmol/（mgpro·30 min） ; The values of Km were 0.4171 mmoL/L, 0.4374 mmol/L; The values of Vmax were 0.8525 nmol/（mgpro·min）, 0.4075 nmol/（mgpro＇min） respectively. Conclusion /3. germanica in Nanchang developed resistance to most of the tested insecticides but propoxur, and the resistance was associated with the changes of biochemical activities of the CarE.

关 键 词：德国小蠊 抗药性 羧酸酯酶 

分 类 号：R384.9[医药卫生—医学寄生虫学] S481.4[医药卫生—基础医学]