小鼠E-W核神经元中磷酸化ERK1/2在异氟醚麻醉-苏醒过程中的表达变化  

ALTERATION OF PHOSPHORYLATED ERK1/2 IN THE NEURONS OF THE EDINGER-WESTPHAL NUCLEUS IN MICE DURING ISOFLURANE ANESTHESIA-RECOVERY CYCLE

作　　者：朱正华[1] 宋乐 张西京[1] 段小莉[3] 熊利泽[1] 王百忍[3] 

机构地区：[1]第四军医大学西京医院麻醉科 [2]广州军区武汉总院神经内科,武汉430070 [3]第四军医大学神经科学研究所,西安710032

出　　处：《神经解剖学杂志》2007年第4期390-394,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(Nos.30600582;30471663)资助项目

摘　　要：为了观察小鼠脑内E-W核神经元中磷酸化的ERK1/2(pERK1/2)在异氟醚吸入麻醉-苏醒过程中的表达变化,为探讨E-W核在麻醉效应产生机制中的作用提供形态学证据。我们将36只8周龄雄性BALB/c小鼠随机分为6组。第1组为清醒对照组(Con);第2、3组为异氟醚麻醉组(Iso-1、Iso-2:分别吸入1.0MAC异氟醚5min和1h);4～6组为异氟醚麻醉苏醒组(W-1、W-2:吸入1.0MAC异氟醚5min后停药2min、30min;W-3:吸入1.0MAC异氟醚1h后停药30min)。用免疫组织化学方法(ABC法)观察各时间点E-W核内pERK1/2阳性细胞并计数;用荧光双重标记法进一步明确pERK1/2阳性神经元的性质。结果显示:正常清醒小鼠E-W核内pERK1/2阳性细胞数量很少(2.2±1.5);异氟醚麻醉过程中pERK1/2表达显著增高(阳性细胞计数,Iso-1∶40.9±8.1;Iso-2∶40.2±9.6,与清醒对照相比,P<0.001);苏醒30min时pERK1/2表达降至正常对照组水平(W-2∶2.1±2.2;W-3∶0.75±1.2)。pERK1/2阳性神经元部分呈促肾上腺皮质激素释放激素(CRF)阳性,部分是乙酰胆碱(ChAT)阳性。上述结果提示,异氟醚麻醉过程中小鼠脑内E-W核神经元被激活,ERK1/2信号通路可能通过兴奋CRF能和Ach能神经元对瞳孔反射及麻醉应激效应进行调控。To investigate the alteration of phospho-ERK1/2 in the neurons of the Edinger-Westphal nucleus during anesthesia-recovery cycle by isoflurane inhalation. Thirty-six BALB/c male mice of 8 weeks old were randomly divided into six group ： control group （ Con, n = 6）, anesthesia groups （Iso-1, Iso-2： received 5min and 1 h inhalation of isoflurane, respectively, n=6 each）, recovery groups （ W-1, W-2： resuming 2 rain and 30 min after 5 rain inhalation of isoilurane, respectively; W-3, resuming 30 rain after 1 h inhalation of isoilurane）, Immunohistochemical staining （ABC method） was used to observe pERK1/2 positive neurons at each time point. Immunofluorescent double-staining of pERK1/2 and CRF or ChAT was performed to definitude the properties of pERK1/2 positive neurons. The results showed that： in control group, only a few of pERK1/2 positive neurons （2.2 ± 1.5 ）were observed in the E-W nucleus; compared to the control group, pERK1/2 positive neurons significantly increased in the E-W nucleus after receiving isoflurane inhalation both in Iso-1 group （40.9 ± 8.1, P 〈 0. 001 ） and Iso-2 group （40.2 ± 9.6, P 〈 0. 001 ）; The expression of pERK1/2 decreased to the normal level （W-2 group, 2.1 ±2.2; W-3 group, 0.75 ± 1.2） at 30 rain after stopping inhalation of isoflurane; some pERK1/2 positive neurons co-localized with CRF, and some with CHAT. These results suggest that pERK1/2 is highly expressed in E-W nucleus and may play a role by modulation of the secretion of CRF or acetylcholine during general anesthesia by isoflurane inhalation.

关 键 词：ERK1/2 磷酸化 E-W核 异氟醚 小鼠 

分 类 号：R614[医药卫生—麻醉学]