一种新型可用于基因传输的非病毒性聚合物载体的合成与表征  

Synthesis and Characterization of a New Non-viral Polymer as a Promising Carrier System for DNA Delivery

作　　者：赵湘云[1] 吴运东[1] 吴育林[1] 刘承斌[1] 向建南[1] 聂书明[1] 

机构地区：[1]湖南大学化学化工学院,湖南长沙410082

出　　处：《生命科学仪器》2007年第7期47-50,共4页Life Science Instruments

摘　　要：一种新型可生物降解聚合物1(chitosan-g-PEI-g-PEG-OH)通过壳聚糖、聚乙烯亚胺以及聚乙二醇的接枝反应合成。采用红外光谱和氢核磁共振谱对其结构进行表征。该接枝聚合物具有双亲性特征,在水溶液中可以自组装形成壳核结构的胶束,经扫描电镜观察该纳米粒子粒径在30-50nm。该聚合物具有非病毒性和可生物降解性,是一种潜在的基因传输载体。A new non-viral conjugate, chitosan-g-PEI-g-PEG-OH polymer, was synthesized using grafting reaction with chitosan, polyethylenimine and polyethylene glycol for constructing amphiphilie grafting eopolymers. The conjugates were characterized by IR and 1HNMR, and can self-assemble to form mieelle-like nanopartieles in the aqueous media at the PH value of 5. The size of the nanopartieles was in the range of 30-50 nm. The hydrotropie polymer mieelles provide an alternative approach for DNA delivery.

关 键 词：壳聚糖 聚乙烯亚胺 聚乙二醇 

分 类 号：O621.3[理学—有机化学]