人促甲状腺激素生物素-亲和素系统免疫放射分析法的建立  

Establishment of immunoradiometric assay for human thyrotropin using biotin-avidin system

作　　者：唐刚华[1,2] 唐国忠[1,2] 杨建忠[1,2] 

机构地区：[1]中国科学院上海原子核研究所 [2]中国医学科学院中国协和医科大学北京协和医院

出　　处：《核技术》1997年第5期267-271,共5页Nuclear Techniques

摘　　要：报道将ＢＡＳ（生物素－亲和素系统）引入双位点夹心ＩＲＭＡ（免疫放射分析法），建立了人血清ＴＳＨ（促甲状腺激素）固相试管双位点夹心ＢＡＳ－ＩＲＭＡ。该法批内ＣＶ为１．４％—８．３％，批间ＣＶ为６．１％—８．４％，最小检出量为０．０４ｍＩＵ／Ｌ，平均回收率为１００．１６％，灵敏度约是同条件下ＩＲＭＡ的３倍；与ＩＲＭＡ对比测定２０份病人血清ＴＳＨ样品，经统计学处理，两者测定结果呈良好相关性。This paper reports the establishment of solid-phase two-antibody sandwich immunoradiometric assay (IRMA) for human thyroid stimulating hormone (hTSH), using the biotin-avidin system (BAS) separation technique. The intraassay CV of the assay is 1.4%—8.3%, the interaassay CV is 6.1%—8.4%, the sensitivity is 0.04mIU/L in serum and the average recovery rate 100.16%. The solid-phase tubes BAS-IRMA has being used to measure 20 serum samples and the results are compared with IRMA. There was a good linear correlation between the results of this method and that of the common IRMA method by statistical analysis. The sensitivity of the former is a little bit better than the common IRMA established under the same condition.

关 键 词：促甲状腺激素 生物素-亲和素 固相试管 

分 类 号：R446.61[医药卫生—诊断学] R817[医药卫生—临床医学]