用^(31)PNMR研究小鼠脑在缺氧状态下能量代谢的动态变化  

DYNAMIC CHANGES IN ENERGETIC METABOLISM OF MOUSE BRAIN IN HYPOXIA STUDIED BY ^(31)P INVIVO NMR METHOD

作　　者：颜贤忠[1] 晏礼明[2] 徐家鸽 杜泽涵[1] 吴本鸽 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850 [2]北京医科大学生物物理系,北京100083

出　　处：《波谱学杂志》1997年第2期147-152,共6页Chinese Journal of Magnetic Resonance

摘　　要：用31P体内核磁共振方法无损伤地观察了C57小鼠脑在缺氧时与能量代谢有关的含磷化合物Pi，PCr，ATP的动态变化，当气体中氧气含量降到5％时，小鼠大脑的PCr，ATP逐渐减少，Pi不断增加，脑组织内pH值降低至6．83～6．93．当Pi与PCr的峰高比等于1时给予复氧，发现复氧时Pi立即降低且pH立即升高的小鼠可以恢复，反之则不可恢复，表明Pi和pH反映了大脑缺氧损伤程度．Changes of phosphates related to energetic metabolism such as PCr, Pi, ATP in C57 mouse brainin hypoxia were observed noninvasively by 31P in vivo NMR method. When the content of oxygen wasreduced to 5%, the concentrations of PCr, ATP decreased while Pi increased, pH of brain cell decreasedto 6.83 ~ 6.93. Mice were reoxygenated when the ratio of peak heights of Pi to PCr were about 1.Those that the ratio of pi after and before reoxygenation were larger than 1 and pH in reoxygenationincreased immediately, could be recovered, otherwise couldn't be recovered. The results showed thatPi and pH reflect the degree of brain damage in hypoxia.

关 键 词：磷31 NMR 能量代谢 缺氧状态 脑缺氧 

分 类 号：R816.1[医药卫生—放射医学]