山莨菪碱合成品的毛细管电泳手性拆分及其组成分析  

Enantiomeric Separation and Composition Analysis of Synthetic Anisodamine by CE

作　　者：林梅[1] 张正行[2] 陈怡[3] 范国荣[3] 安登魁[2] 

机构地区：[1]上海市食品药品检验所,上海201203 [2]中国药科大学药学院,江苏南京210009 [3]第二军医大学药学院,上海200433

出　　处：《中国医药工业杂志》2007年第8期580-583,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了毛细管电泳法手性拆分山莨菪碱的四个光学异构体。采用未涂层石英毛细管,75mmol/L磷酸盐为运行缓冲液(pH2.5,含25mmol/L羧甲基-γ-CD),运行电压20kV,毛细管柱温25℃,电迁移进样8kV×6s,检测波长200nm。测定了山莨菪碱合成品两对对映异构体的组成,10个批号中两对对映异构体的组成比例约为3∶2。A capillary electrophoresis method was established for chiral separation of four anisodamine stereo isomers. An uncoated capillary column was used, with 75 mmol/L phosphate buffer solution （pH 2.5, containing 25mmol/L carboxymethylated-γ-CD） as running buffer, at 20 kV applied voltage. The capillary column temperature was 25℃ and the injection condition was 8 kV×6 s by electrokinetic mode. The on-line detection wavelength was 200nm. The proportion of two pairs of enantiomers in ten samples of synthetic anisodamine was 3 ： 2.

关 键 词：山莨菪碱 电泳 手性拆分 组成分析 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.8[化学工程—制药化工]