新城疫病毒TL1株M基因的克隆和序列分析  

Genetic variation of matrix protein gene in TL1 strain of Newcastle disease virus

作　　者：王学理[1] 王兴龙 李晓艳 刘锴[1] 任林柱[1] 崔丽瑾[1] 闫广谋[1] 段小宇[1] 

机构地区：[1]解放军军事医学科学学院军事兽医研究所

出　　处：《畜牧与兽医》2007年第5期8-11,共4页Animal Husbandry & Veterinary Medicine

基　　金：军事医学科学院科技创新基金资助项目;吉林省科技厅科技发展计划项目(20050549)

摘　　要：采用RT-PCR方法扩增了新城疫病毒（NDV）TL1株M基因，将扩增产物提纯后克隆入pGEM-Teasy载体，通过酶切、PCR和测序验证克隆正确。测序拼接得出M基因的序列长度为1232bp，该基因的ORF总长为1095bp，编码364个氨基酸。与GenBank下载的15株参考毒株M基因比较，核苷酸同源性为85．2％～98．1％，编码的氨基酸同源性为85．8％～98．9％。NDVTL1产生M蛋白分子中有7对碱性氨基酸，5个保守的Cys残基，与鹅源新城疫SF02株、ZJ1株具有类似的特征。这些结果为揭示NDVTL1株的分子生物学背景，阐明NDV种间传播机制奠定了基础。Gene M of Newcastle disease virus （NDV） TL1 strain was amplified by RT-PCR, cloned to pGEM-T easy vector, sequenced and analyzed. The complete nucleotide sequence of gene M is 1232 bp long. The major open reading frame （ORF） of 1095 bp contains a coding region of 364 amino acid residues. A comparison of gene M sequences in the TL1 strain with other 15 strains of NDV is 85.2 % to 98. 1% identity at nucleotide and 85. 8 % to 98.9 % identity at amino acid. Seven pairs of basic amino acids and 5 conservative Cys residues show similar characterization in ZJ1, SF02 and TL1 strains of Newcastle disease virus. These results have indicated the molecule biological background of NDV TL1 strain and provided groundwork to understand mechanisms of molecule evolution and its genetic and variation of NDV.

关 键 词：新城疫病毒 M基因 遗传变异 

分 类 号：S855[农业科学—临床兽医学]