还原型谷胱甘肽对罗非鱼生长和抗氧化性能的影响  

The Effects of Reduced Glutathione on Growth Rate and Antioxidation Capacity of Tilapia

作　　者：张国良[1] 赵会宏[1] 周志伟[1] 何志交[1] 郭慧[1] 陈静[1] 刘丽[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《华南农业大学学报》2007年第3期90-93,共4页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2004B20301007);农业部渔业生态环境重点开放实验室开放基金课题"谷胱甘肽(GSH)对罗非鱼生长性能及免疫功能影响的研究"

摘　　要：将体长、体质量相近的奥尼罗非鱼Oreochromis niloticus×O.aureus360尾,随机分为4组,每组3个重复,每个重复30尾.在Ⅰ、Ⅱ、Ⅲ、Ⅳ组的日粮中分别添加0、100、200、400 mg/kg剂量的还原型谷胱甘肽(GSH),试验期60 d,检测了与生长性能、血清中类胰岛素样生长因子-I(IGF-I)水平及肌肉组织抗氧化能力相关的各项指标.结果表明:1)在试验期间,添加GSH的Ⅱ组和Ⅳ组的相对体质量生长率(RSGR)显著高于对照组Ⅰ组(P<0.05),而且第20 d时,Ⅱ组和Ⅳ组鱼体的平均体质量显著高于Ⅰ组(P<0.05);2)第40 d和第60 d,Ⅳ组鱼体的血中IGF-I水平均显著高于Ⅰ组(P<0.05);3)第20 d,各处理组鱼体肌肉组织的总抗氧化能力(T-AOC)显著高于Ⅰ组(P<0.05).第40 d,Ⅳ组的T-AOC和总超氧化物歧化酶(T-SOD)活力均显著高于Ⅰ组(P<0.05).第60 d,Ⅱ组的T-AOC和T-SOD活力显著高于Ⅰ组(P<0.05).以上结果显示,在饲粮中添加适宜剂量的GSH能改善鱼体肌肉组织的抗氧化能力,并使IGF-I水平在正常生理范围内有所升高,从而对鱼体的生长起促进作用.The effects of reduced glutathione （GSH） as a feed additive on tilapia growth, serum hormones and antioxidation capacity were studied. Three hundred sixty fish （ Oreochromis niloticus x O. aureus ） were assigned randomly to four groups. Three groups were fed ration with 100,200 and 400 mg/kg GSH additive, respectively, for 60 d. The other group served as a control fed ration without the additive. Resuits showed that during the feeding trial, RSGR of fish fed 100 and 400 mg/kg additive were statistically .greater than that in the control group（P 〈0.05）, respectively. In particular, at 20 d which is the onset of the feeding trial, body mass in those two groups were respectively statistically greater than that in the control group（ P 〈0.05 ）. At 40 and 60 d, serum IGF-I levels in fish fed 400 mg/kg additive were statistically higher than that in the control group（ P 〈 0.05 ）. At 20 d, fish fed GSH had higher T-AOC than that in the control group（ P 〈 0.05 ）. At 40 d, fish fed 400 mg/kg additive had statistically higher T-SOD activity and T-AOC than those in the control group（ P 〈 0.05 ）, and so did fish fed 100 mg/kg additive at 60 d. These data indicated that administration of GSH in feed could improve fish muscle tissue antioxidation ability, increase hormone levels and metabolism, positively affecting fish growth.

关 键 词：罗非鱼 还原型谷胱甘肽 生长 抗氧化性能 

分 类 号：Q591.2[生物学—生物化学]