甲硝唑半抗原及全抗原合成与鉴定  

Synthesis and Identification of the Hapten and Complete Antigen of Metronidazole

作　　者：雷红涛[1] 沈玉栋[1] 刘文字[2] 邱志超[1] 杨瑾[1] 孙远明[1] 

机构地区：[1]广东省高等学校食品质量安全重点实验室华南农业大学食品质量安全研究所,广东广州510642 [2]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2007年第3期101-104,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金(20377016);广东省自然科学基金(032240);粤港关键领域重点突破项目(2006A25005002)

摘　　要：在微波加热条件下,将甲硝唑与琥珀酸酐反应,合成半抗原甲硝唑半琥珀酸酯,氢核磁共振、电喷雾质谱、红外光谱方法的鉴定结果表明,目标半抗原合成成功;通过活泼酯法将半抗原与载体蛋白偶联制备甲硝唑全抗原,紫外光谱法鉴定结果表明,全抗原合成成功.The hapten metronidazole monosuccinate was synthesized by reacting metronidazole and succinic anhydride under microwave heating. Identification using 1^H nuclear magnetic resonance, electrospray ionization mass and infrared spectrum showed the hapten was successfully synthesized. Then active ester method was used to produce complete antigen of metronidazole, which was confirmed by analysis using ultraviolet spectrum.

关 键 词：甲硝唑 半抗原 全抗原 鉴定 

分 类 号：S859[农业科学—临床兽医学]