粉红黏帚霉67-1菌株寄生核盘菌菌核的酶学动态研究  

Dynamic Studies of Some Cell Wall Degrading Enzymes Involved in Mycoparasitism of Gliocladium roseum 67-1 on Sclerotia of Sclerotinia sclerotiorum

作　　者：李晶晶[1] 张拥华[2] 陈巧云[2] 李世东[2] 谢响明[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]中国农业科学院植物保护研究所,北京100081

出　　处：《菌物研究》2007年第2期107-109,共3页Journal of Fungal Research

基　　金：国家"863"项目(2006AA10A211)

摘　　要：对粉红黏帚霉67-1菌株侵染核盘菌菌核过程的多种细胞壁降解酶活性进行了连续测定,以研究几丁质酶等在这一寄生互作体系中的可能作用。结果表明:葡聚糖酶活性变化表现活跃,且随寄生过程呈增加趋势,配对法T检验结果表明,第10d的处理与对照酶活性差异达到最大;几丁质酶、蛋白酶活性变化表现较低,而纤维素酶未检测得到。酶学动态变化与之前石蜡切片显微观察的结果在时间上表现一致;认为葡聚糖酶可能是粉红黏帚霉67-1菌株寄生核盘菌菌核的关键酶。Gliociadium spp. ,a kind of fungi broadly habited in soil,was one important bio-control factor of plant diseases. Previous studies indicated that G. roseum 67-1 parasited and destroyed many plant pathogenic fungi. Here reported are some results of the study of the key enzymes involved in the mycoparasitism of G. roseum 67-1 on Sclerotinia sclerotiorum. Dynamic analysis of the activities of cell wall degrading enzymes showed that the activity of glucanase increased evidently companied with the mycoparasitism process and might play a great role. Meanwhile, the activities of chitinase and proteinase behaved lower and the cellulose activity even could not be detected.

关 键 词：粉红黏帚霉 核盘菌 菌核 重寄生 细胞壁降解酶 

分 类 号：Q949.331[生物学—植物学]