蛋白酪氨酸磷酸酶1B在3T3-L1前脂肪细胞分化过程中表达的变化  

Expression changes of protein tyrosine phosphatase 1B during differentiation of 3T3-L1 preadipocytes

作　　者：陈月[1] 邹大进[1] 张征[1] 王淼[1] 吴捷[1] 

机构地区：[1]第二军医大学长海医院内分泌科,上海200433

出　　处：《第二军医大学学报》2007年第8期838-842,共5页Academic Journal of Second Military Medical University

摘　　要：目的:观察在3T3-L1前脂肪细胞分化过程中蛋白酪氨酸磷酸酶1B(PTP1B)mRNA表达及其蛋白水平的变化,探讨PTP1B与脂肪细胞分化的关系。方法:体外培养3T3-L1前脂肪细胞,在3T3-L1前脂肪细胞分化成熟过程中(第1～10日),采用油红O染色及RT-PCR法检测过氧化物酶体增殖物活化受体β2(PPARγ2)基因表达评价脂肪细胞分化成熟情况,RT- PCR法及Western印迹法检测脂肪细胞分化过程中PTP1B mRNA表达及其蛋白水平的变化。结果:随着脂肪细胞诱导分化逐步深入,油红O染色显示其中脂滴形成逐渐增多,至占全视野90%以上;同时PPARγ2基因表达逐步增多,均提示脂肪细胞分化逐步成熟,至最终分化为成熟脂肪细胞。PTP1B mRNA和蛋白水平在前脂肪细胞中表达较高,随着脂肪细胞的逐步成熟,其表达逐渐降低,至分化成熟时表达水平降至最低。结论:在3T3-L1前脂肪细胞分化成熟过程中,PTP1B mRNA及其蛋白表达水平逐渐减低,PTP1B可能参与了脂肪细胞分化。Objective：To observe the expression changes of protein tyrosine phosphatase 1B （PTPIB） mRNA and protein during the differentiation of 3T3-L1 preadipocytes, so as to explore the relationship between PTP1B and adipocytes differentiation. Methods： 3T3-L1 preadipocytes were cultured in vitro and were induced to differentiate into mature adipocytes; the differentiation of adipocytes was assessed through detecting expression of peroxisome proliferator-activated receptor-gamma 2 （PPAR72） mRNA by RT-PCR and oil red O staining. Expression of PTPIB in adipocytes was detected by RT-PCR and Western blot during differentiation. Results： With the progression of 3T3-L1 cell differentiation, oil red O staining showed that the lipid droplets increased gradually to 90 % of the vision field; meanwhile, the expression of PPAR）＇2 also increased gradually, suggesting the proliferation and maturation of the preadipocytes. The expression of PTPIB mRNA and protein decreased in 3T3-L1 preadipocytes with their differentiation and maturation; the expression reached the lowest level in mature adipocytes, Conclusion.. Expression of PTPIB mRNA and protein decreases during the differentiation of preadipocytes, indicating a role for PTPIB in the maturation of adipocytes.

关 键 词：蛋白质酪氨酸磷酸酶 3T3-L1细胞 细胞分化 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]