阿尔泰狗哇花总黄酮和总皂苷定量分析方法研究  

Determination of Total Flavnoids and Triterpenoidal Saponins in Heteropappus altaicus(Willd.) Novopokr.by UV-Vis Spectroscopy

作　　者：杨淑敏[1] 赵青岚[1] 李明静[1] 赵东保[1] 刘绣华[1] 

机构地区：[1]河南大学化学化工学院,河南开封475001

出　　处：《河南大学学报（自然科学版）》2007年第4期358-360,共3页Journal of Henan University:Natural Science

基　　金：河南省教育厅科技攻关项目(2006150032)

摘　　要：采用分光光度法,以槲皮素为对照品经AlCl3-NaAc显色测定总黄酮含量,以齐墩果酸为对照品经香草醛-高氯酸显色测定总皂苷含量.分析结果表明,原药材中总黄酮和总皂苷的含量分别为17.79和40.86 mg/g,线性范围分别为30.6～112.2μg(r=0.999 7)和20～60μg(r=0.999 7),平均回收率分别为97.7%(RSD=2.4%,n=5)和98.7%(RSD=0.98%,n=5).该法简便、准确、灵敏度高、重现性好,也可用于阿尔泰狗哇花总黄酮和总皂苷产品的含量测定.To determine the total flavonoids and triterpenoidal saponins in Heteropappus altaicus （Willd.） Novopokr. by UV-Vis. Quercetin and oleanolic acid were used as the reference compounds respectively. The calibrations for total flavnoids and triterpenoidal saponins showed good linearity over the ranges of 30.6- 112.2 μg （r=0.999 7） and 20-60 μg（r=0.999 7）, respectively. The recoveries were 97.7 % （RSD=2.4%, n=5） for total flavnoids and 98.7 % （RSD=0.98 %, n=5） for triterpenoidal saponins. The methods were practical, rapid, accurate, and sensitive. We got satisfactory results when determined flavnoids and triterpenoidal saponins in Heteropappus altaicus （Willd.） Novopokr. using the methods.

关 键 词：阿尔泰狗哇花 总黄酮 总皂苷 紫外-可见分光光度法 

分 类 号：O174.2[理学—数学]