母猪FcRn基因多态性及其对初乳中IgG含量的影响  

Polymorphism of FcRn Gene and Its Effects on the Sow Colostrum IgG Concentration

作　　者：王炳艳[1] 王立贤[2] 王婷[3] 翟丽维[1] 王楚端[1] 李振宽[3] 周伟良[3] 

机构地区：[1]农业部畜禽遗传育种重点开放实验室 [2]中国农业科学院北京畜牧兽医研究所,北京100094 [3]天津市宁河原种猪场,天津301504

出　　处：《畜牧兽医学报》2007年第8期765-768,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划高繁殖力瘦肉型猪新品种选育(2006BAD01A08)

摘　　要：对猪IgG C片段受体基因(FcRn)的单链构象多态性进行分析,在FcRn基因Site1位点即第3外显子的147处碱基发现T突变为C和在Site6位点即第6外显子的1 040处碱基发现C突变为G,其中外显子6的碱基突变使苏氨酸变为精氨酸。在3个品种207头母猪样本中检验多态位点和初乳中IgG含量的相关性,建立固定效应模型进行最小二乘分析,对不同基因型的初乳中IgG含量进行差异显著性检验。结果表明:试验群体中Site1位点的突变对初乳中IgG含量没有显著影响(P>0.05),Site6位点的突变对初乳中IgG含量有显著影响(P<0.05),多重t检验结果表明Site6位点不同基因型的初乳中IgG含量差异显著(P<0.05):CC型的初乳中IgG含量最高,DD型的初乳中IgG含量最低,说明FcRn基因可以作为猪初乳中IgG含量的候选基因之一。The polymorphism of the FcRn gene was analyzed, and two SNPs were found by the technique of single strand conformation polymorphism （SSCP） and finally confirmed by sequencing. The frequencies of different genotypes in 207 sows of the three breeds were calculated and the relationship between three genotypes and IgG Concentration in Colostrum of the Sow were analyzed based on liner model. The results showed that the mutation in the Sitel did not have effect on IgG concentration in colostrum of the sows（P〉0.05） , the mutation in the Site6 had effect on IgG concentration in colostrum of the sows （ P〈0. 05） ; Multiple t comparisons in site6 indicated that IgG concentration in colostrum of the sows between CC,CD and DD genotype were significant（P〈0.05） ：IgG concentration in colostrum of the sows of CC is the highest, while that of the sows of DD is the lowest. The result indicated FcRn gene could be a candidate gene of IgG concentration in colostrum of the sows.

关 键 词：猪 FcRn基因 单核苷酸多态 初乳 IGG 

分 类 号：S828.2[农业科学—畜牧学]