大豆苷元对乳鼠心肌细胞缺氧/复氧损伤的保护作用  

The Protective Effect of Daidzein on Cardiomyocytes of Neonatal Rats Injuried by Anoxia/Reoxygenation

作　　者：许大庆[1] 雷婕[1] 彭晓东[2] 林蓉[3] 

机构地区：[1]宁夏医学院第二附属医院药剂科,银川750001 [2]宁夏医学院基础学院药理学教研室,银川750004 [3]西安交通大学医学院药理学教研室,西安710000

出　　处：《宁夏医学院学报》2007年第4期344-346,共3页Journal of Ningxia Medical College

摘　　要：目的观察大豆苷元对乳鼠心肌细胞损伤的影响并探讨其机理。方法体外培养乳鼠心肌细胞，制备缺氧／复氧损伤模型，测定细胞存活率、上清液中乳酸脱氢酶（LDH）活性、肌酸激酶（CK）、丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）和一氧化氮合酶（NOS）含量。结果大豆苷元10-160μmol／L均能提高缺氧／复氧损伤心肌细胞的存活率和用MTT法测得的A570nm值（P〈0．01）；抑制缺氧／复氧损伤引起心肌细胞的LDH和CK释放；减少MDA的生成，提高SOD的活性，抑制NOS活性，降低NO水平（P〈0．05，P〈0．01）。结论大豆苷元对乳鼠缺氧／复氧损伤的心肌细胞具有保护作用。Objective To observe the protective effects of daidzein on the myocardial cells injured by Anoxia/ reoxygenation（A/R） and to explore its action mechanisms with the method of cell culture. Methods Myocardial cells from 2-3 days rats were cultured in the 1640 medium and the cellular injury was induced by A/R. The viability of myocardial cells, dehydrogenase （LDH）, creatine kinase （ CK ）, malonaldehyde （MDA）, surperoxide dismutase（SOD）,nitric oxide（NO）and nitroxide synthase（NOS） in the medium were measured.Results Daldzein （ 10 - 160μmol/L） significantly increased the viability of myocardial cells and A570um of MTT, inhibited release of LDH and CK, decreased the content of MDA and increased SOD activity, decreased the contents of NO and NOS of myocardial cells injured by A/R（ P 〈 0.05, P 〈 0.01 ）. Conclusions daidzein has the protective effects on myocardial cell injury and the effects are beneficial to prevention and treatment of Myocardial reperfusion injury（MRI）.

关 键 词：大豆苷元 乳鼠 心肌细胞 缺氧/复氧损伤 

分 类 号：R285.5[医药卫生—中药学]