多重置换扩增——一种新的全基因组扩增技术  

Multiple Displacement Amplification （MDA）--A New Whole Genome Amplification （MGA） Method

作　　者：刘维瑜[1] 金春莲[1] 

机构地区：[1]中国医科大学基础医学院遗传教研室,沈阳110001

出　　处：《国际遗传学杂志》2007年第4期265-268,共4页International Journal of Genetics

基　　金：“十五”国家科技攻关课题（2004BA720A04）

摘　　要：全基因组扩增技术（MDA）用于扩增大量的DNA以满足遗传检测的需要。在高通量的遗传分型中，处理有限的临床样本的基因组DNA，一直是个瓶颈问题。一种新方法——多重置换扩增，能高度忠实的复制整个基因组DNA，扩增出10～100kb大小的片段，能提供大量均一完整的全基因组序列。MDA是一种简单、有效的方法，非常适用于遗传研究、法医学和临床诊断的需要。Whole genome amplification methods are used to generate the large amounts of DNA that are required for genetic testing. Preparation of genomic DNA from clinical samples is a bottleneck in high- throughput genotyping and is frequently limited by the amount of specimen available. A new method, called multiple displacement amplification, dramatically improves the high-fidelity amplification of genomic DNA, with 10-100 kb amplified DNA products large amount of genome sequence . MDA-based WGA is a simple and reliable method that may have significant implications for genetic studies, forensic medicine and clinical diagnostics.

关 键 词：全基因组扩增 多重置换扩增 phi29 DNA聚合酶 

分 类 号：Q[生物学]