降低PARP酶活性对外源基因稳定整合的影响  

Effects of Lowering PARP Enzyme Activity on the Stable Integration of the Exogenous Gene

作　　者：杨其伟[1] 陈雅文[1] 史国利[1] 陈德风[1] 张自立[2] 

机构地区：[1]南开大学分子生物学研究所 [2]南开大学生化及分子生物学系

出　　处：《遗传》1997年第3期1-4,共4页Hereditas（Beijing)

基　　金：国家自然科学基金

摘　　要：通过使用ＰＡＲＰ酶的抑制剂研究了降低ＰＡＲＰ酶的活性对外源基因转染效率及整合作用的影响，结果表明，转染的外源基因的吸收及瞬时表达不依赖于ＰＡＲＰ酶的活性，而外源基因的整合作用与ＰＡＲＰ酶的活性有关。In this study, the effect of lowering PARP activity on the transfection of cultured cells with foreign genes was evaluated. The results indicated that PARP enzyme involved in the stable integration of foreign genes into the host genome. However, inhibition of PARP activity exhibited no effect on both the uptake into the cells and the expression of the foreign genes.

关 键 词：聚腺苷二磷酸 核糖聚合酶 PARP酶 外源基因 整合 

分 类 号：Q75[生物学—分子生物学]