双歧杆菌DNA对巨噬细胞MAPK和PKC以及NF-kB的影响(英文)  

Influence of Bifidobacterium DNA on MAPK,PKC and NF-kB of macrophages

作　　者：王立生[1] 李迎雪[1] 朱惠明[1] 朱忠生[1] 马晓东[2] 

机构地区：[1]暨南大学第二临床医学院消化科,广东深圳518020 [2]南方医科大学中心实验室,广东广州510515

出　　处：《中国现代医学杂志》2007年第15期1799-1803,1810,共6页China Journal of Modern Medicine

基　　金：two grant from the Natural Science Foundation of Guangdong Province(No.04006961;No.5009077)

摘　　要：目的以信号分子MAPK家系、PKC家族和NF-kB为线索探索青春型双歧杆菌的DNA激活巨噬细胞的途径。方法以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞ERK1/2、JNK、p38、PKCα、PKCβI、PKCβⅡ、PKCγ、PKCε和PKCζ的含量。以细胞免疫化学方法检测巨噬细胞NF-kB的阳性细胞密度。结果双歧杆菌DNA注射组小鼠腹腔巨噬细胞ERK1/2、PKCα和PKCβⅡ的平均荧光强度明显高于对照组(P<0.01),而JNK、p38、PKCβI、PKCγ、PKCε和PKCζ的平均荧光强度在两组间则差异无显著性(P>0.05)。双歧杆菌DNA注射组巨噬细胞NF-kB的阳性细胞密度显著高于对照组(P<0.01)。结论青春型双歧杆菌的DNA可通过活化ERK1/2、PKCα、PKCβⅡ和NF-kB来激活巨噬细胞。[Objective] To explore the pathway of DNA of Bifidobacterium adolescence activating macrophages in view of MAPK family, PKC family and NF-κB. [Methods] The contents of ERK1/2, JNK, p38, PKC α, PKC β Ⅰ, PKC β Ⅱ, PKC γ PKCeand PKC ζ of mouse peritoneal macrophages were detected quantitatively by using laser confocal microscope. The positive cell density of NK-κB of macrophages was detected by employing immunocytochemistry method. [Results] The average fluorescent strength of ERK1/2, PKC α and PKC β Ⅱ produced by mouse peritoneal macrophages in Bifidobacterium DNA injection group was markedly higher than that in control group（P 〈 0.01 ）. The average fluorescent strength of JNK, p38, PKC β Ⅰ, PKC , PKCγ and PKC ε did not have significant differences between the two groups（P 〉0.05）. The positive cell density of NK-κB of macrophages in Bifidobacterium DNA injection group was markedly higher than that in control group（P 〈0.01 ）. [Conclusion] DNA of bifidobacteriua adolescence could activate macrophages by promoting the activity of ERK1/2, PKC α, PKC β Ⅱ and NF-κB.

关 键 词：双歧杆菌DNA 巨噬细胞 丝裂素活化的蛋白激酶 蛋白激酶C 核因子-κB 

分 类 号：R378.992[医药卫生—病原生物学]