对氧磷酶启动子基因多态性与2型糖尿病合并冠心病的关系  

Study on ralationship between paraoxonase-1 and type 2 diabetes mellites complicated by coronary heart disease

作　　者：邵海琴[1] 秦冰[1] 张英[2] 

机构地区：[1]河北工程大学医学院检验系,河北邯郸056002 [2]武汉市第一医院肿瘤科,湖北武汉475000

出　　处：《中国现代医学杂志》2007年第15期1841-1843,1846,共4页China Journal of Modern Medicine

摘　　要：目的探讨中国人对氧磷酶(PON1)基因-108C/T遗传多态性与2型糖尿病(T2DM)合并冠心病(CHD)的关系。方法应用多聚酶链反应(PCR)-限制性片段长度多态性(RFLP)法分析PON1基因-108C/T遗传多态性在T2DM合并CHD组、单纯T2DM组、正常对照组的基因频率。结果中国人群PON1-108C/T基因位点C、T等位基因频率分别为0.477和0.523,是一个普遍多态性,T2DM合并CHD组患者T等位基因频率明显高于单纯T2DM组、正常对照组(P<0.05)。结论PON1-108C/T多态性位点T等位基因与2型糖尿病合并冠心病相关联。[Objective] To study the association between the polymorphism in the promoter region in human paraoxonase （PON1） gene and type 2 diabetes mellitus （T2DM） complicated by coronary heart disease （CHD） in Chinese population. [Methods] The genotype and allele frequency of paraoxonasel gene polymorphisms were assayed by polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP）. [Results] C and T allele frequency of PON1 in control was 0.477 and 0.523 respectively. It was universal polymorphism of PONI-108C/T. T allele frequency had significant difference between T2DM complicated by CHD group and T2DM and control （P 〈 0.05）. [Conclusion] T allele frequency of PON1-108C/T maybe associate with T2DM complicated by CHD in Chinese population.

关 键 词：对氧磷酶 基因多态性 2型糖尿病 2型糖尿病合并冠心病 

分 类 号：R587.1[医药卫生—内分泌]