组织芯片和普通切片检测乳腺癌ER、PR、nm23的比较  

A comparative study on the expression of ER,PR and nm23 in breast carcinoma by tissue microarray with usual section

作　　者：梁莉萍[1] 赵峰[1] 郭云泉[1] 房新志[1] 柴敏秀[1] 

机构地区：[1]新疆医科大学附属肿瘤医院病理科,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2007年第7期729-731,共3页Journal of Xinjiang Medical University

摘　　要：目的:探讨组织芯片技术在乳腺癌相关基因产物检测中的应用价值。方法:采用免疫组织化学PV-9000两步法和组织芯片技术,检测150例乳腺浸润性导管癌组织中ER、PR、nm23的表达,并与普通切片免疫组化结果进行比较分析。结果:组织芯片免疫组织化学染色ER、PR、nm23的阳性率分别为31.94%、26.57%、73.61%。普通切片免疫组化染色EP、PR、nm23的阳性率分别为36.81%、29.37%、75.00%。2种免疫组化染色结果比较差异无统计学意义(P>0.05)。结论:组织芯片技术有良好的科研应用价值和发展前景。Objective： To investigate the value of tissue microarray on the examine of associated genes production in breast carcinoma. Methods： ER, PR , nm23 were detected in 150 cases of breast invasive ductal carcinoma by tissue microarray and usual section with immuno-histochemistry technology. Results： The positive rate of ER, PR and nm23 by tissue microarray were 31. 94 %, 26.57 %, 73. 61%, respectively. The positive rate of ER, PR and nm23 by usual section were 36.81%, 29. 370%, 75.0%, respectively. The positive rates of ER, PR and nm23 had significant difference between the usual section immunohistochemistry staining and the tissue microarray immunohistochemistry staining （P 〉0.05）. Conclusion： The tissue microarray has important practical significance and broad application prospect in scientific study.

关 键 词：乳腺癌 组织芯片 免疫组织化学 ER PR NM23 

分 类 号：R392.11[医药卫生—免疫学] R739.9[医药卫生—基础医学]