补体C5b-9复合物促进树突状细胞成熟  

Terminal complement complex C5b-9 promotes maturation of human monocyte-derived dendritic cells

作　　者：杨承英[1] 陈永文[1] 傅晓岚[1] 费蕾[1] 靳廼斯 谢谆怡[1] 汤玉渝[1] 吴玉章[1] 

机构地区：[1]第三军医大学免疫学研究所,重庆400038

出　　处：《现代免疫学》2007年第4期316-321,共6页Current Immunology

基　　金：国家自然科学基金(30600546;30571700)

摘　　要：探讨补体C5b-9复合物对树突状细胞成熟及免疫学功能的影响。rhGM-CSF+rhIL-4体外诱导单核细胞分化为不成熟树突状细胞,体外于不成熟树突状细胞表面用补体蛋白纯品组装CSb-9,37℃,5%CO_2温育96 h,流式分析细胞表型及抗原捕获能力;与同种异体CD4^+和CD8^+T细胞共培养检测其免疫刺激功能;ELISA检测细胞因子分泌。结果显示,亚溶解型C5b-9处理DC表面标志CD83、HLA、CD80、CD86、B7-H1、B7-H3、B7-H4以及BTLA等表达上调;DC分泌IL-12及TNF-α上调,抗原捕获能力降低;C5b-9处理DC刺激CD4^+T细胞活化及分泌IFN-γ、IL-2能力增强。结论:补体C5b-9复合物可以促进树突状细胞成熟,衔接天然免疫和特异性免疫。To study the effects of terminal complement complex C5b-9 on maturation and function dendritic cells in vitro, immature dendritic cells were induced from monocytes with rhGM-CSF and rhIL-4. C5b-9 treated immature dendritic cells were incubated for 96 hours, and then the immune phenotype and antigen uptake activity were determined by using flow cytometry. T cell stimulating activity was analyzed by allo-MLR. Cytokines were analyzed by ELISA. Compared with the control, cell surface marker of C5b-9 treated DC, such CD83, HLA, CD80, CD86, B7-H1, B7-H3, B7-H4, BTLA was significant up-regulated. Cytokine secretion was increased while antigen uptake activity was decreased. The activity of promoting CD4^＋T cells activation and proliferation was increased, at the same time IL-2 and IFN-γ, secretionwas promoted. It is concluded that terminal complements complex C5b-9 link innate and adaptive immune responses through promoting DC maturation.

关 键 词：补体终末复合物C5b-9 树突状细胞 成熟 亚溶解型C5b-9 

分 类 号：R392[医药卫生—免疫学]