乙型肝炎病毒前C区和BCP区突变及基因型对HBeAg表达的影响  

Affection of the mutations and genotypes of hepatitis B virus precore and basic core promoter on HBeAg expression

作　　者：侯远沛 刘成永[1] 高玉金[1] 

机构地区：[1]徐州市传染病医院,江苏徐州221004

出　　处：《临床肝胆病杂志》2007年第4期251-253,共3页Journal of Clinical Hepatology

摘　　要：研究乙型肝炎病毒前C区和BCP区突变及基因型对HBeAg表达的影响。分别用基因芯片和基因测序的方法检验HBV DNA阳性的乙型肝炎病毒前C区和BCP区突变及基因型,应用时间分辨免疫荧光法检测HBeAg含量,按照有无HBV前C区1896、BCP区1762、1764双突变、基因型等指标进行分组分析。无前C区1896和BCP区1762/1764双突变组、单纯1762、1764双突变和1896突变组以及1896、1762、1764联合突变组之间相互比较,HBeAg含量相差显著,F=6.47,P<0.01;B、C基因型间HBeAg含量相比,相差无显著性,t=0.1394,P>0.05。乙型肝炎病毒前C区和BCP区突变能显著影响HBeAg量的表达,从而导致乙型肝炎病毒致病能力的变化。To study the affection of the mutations and genotypes of hepatitis B virus precore and basic core promoter （BCP） on HBeAg expression. Gene chip technique was applied to detect gene mutations in HBV pre -core nt. 1896, BCP nt. 1762 and 1764. And DNA sequencing technique was used to sequence their genotypes Time - resolution immunofluorescenece technique was used to quantitate HBeAg. Those samples were divided according to the conditions mutations and geno- types of precore and BCP. The quantity of HBeAg were significant difference between single 1762/1764 mutation group, single 1896 mutation group, 1896/1762/1764 unite mutations group and no above mutations group （F =6. 47 ,P 〈0. 01 ）. There was no significant different between genotype B group and genotype C group in quantity of HBeAg （t =0. 1394, P 〉 0. 05 ）. Hepatitis B virus precore and BCP mutations might significantly affect the expression of HBeAg, and lead to the variety of HBV pathogenicity.

关 键 词：乙型肝炎病毒 基本核心启动子 前C基因 基因型HBeAg 

分 类 号：R512.62[医药卫生—内科学]