大鼠MODS发病机制探讨  

The study of pathogenesis in rat with MODS

作　　者：谭波[1] 陈媛媛[2] 王兴勇[2] 许峰[2] 

机构地区：[1]重庆市第九人民医院急诊科,重庆400700 [2]重庆医科大学附属儿童医院ICU,重庆400014

出　　处：《重庆医科大学学报》2007年第10期1041-1044,1048,共5页Journal of Chongqing Medical University

基　　金：重庆市卫生局基金资助项目。

摘　　要：目的:探讨MODS大鼠血清IL-6、IL-4和肺泡巨噬细胞表面Ia抗原的变化规律及其之间的关系,阐明促炎/抗炎平衡和免疫状态在MODS发病过程中所起的作用。方法:采用肠缺血-再灌注损伤后12h腹腔注射小剂量酵母多糖的方法,复制MODS动物模型。采用酶联免疫吸附法(EILSA)和流式细胞技术对大鼠血清IL-6、IL-4水平和肺泡巨噬细胞表面Ia抗原的表达进行检测。结果:与假手术对照组比较,模型组大鼠6h后各重要脏器均出现明显病理学改变;随伤后时间的延长,模型组大鼠血清IL-6、IL-4水平逐渐增高,肺泡巨噬细胞Ia抗原表达逐渐降低。结论:MODS时,血清促炎细胞因子(IL-6)和抗炎细胞因子(IL-4)水平均显著增高,早期以促炎反应为主,晚期则以抗炎反应占优势,二者的失衡导致了MODS的发生发展。免疫抑制发生较晚,此时以抗炎反应占优势,IL-6/IL-4比值与免疫抑制的程度密切相关。Objective：To approach the variation and relation of serum IL-6,IL-4 with IaAg on lung alveolus macrophage cell face in MODS rats,to illuminate the roles of proinflammatory/antiinflammatory balance and immune stage in MODS pathogenesis. Methods：MODS rat modes were established by intraperitoneally injected with low-lose zymosan after rats were suhjected to 12 hours of instestine ischemia-reperfusion. Serum levels of IL-6,IL-4 and expressinon of IaAg were determined by Enzyme-Linked immunosorbant assay （ELISA）. Results：Compared with rats of sham-operated group,those of model group showed manifest pathological change in all important organs after 6 hours. Followed by time after injury,serum levels of IL-6, IL-4 in model group were increased gradually. Expression of IaAg on lung alveolus macrophage cell face decreased gradually. Conclusion：During MODS serum levels of proinflammatory cytokine （IL-6） and antiinflammatory cytokine （IL-4） increased significandy. In earlier period, proinflammatory response predominated. In later stage antiinflammatory response prevailed. Disequilibrium of the two resulted in the development of MODS. Immunosupression developed later. At this time antiinflammatory response gained advantage. The ratio of IL-6 to IL-4 was related closedly to the degree of Immunosupression.

关 键 词：MODS IA抗原 IL-6 IL-4 

分 类 号：R392.11[医药卫生—免疫学]